TY - JOUR
T1 - Japanese Encephalitis Virus infection induces IL-18 and IL-1β in microglia and astrocytes
T2 - Correlation with in vitro cytokine responsiveness of glial cells and subsequent neuronal death
AU - Das, Sulagna
AU - Mishra, Manoj Kumar
AU - Ghosh, Joydeep
AU - Basu, Anirban
N1 - Funding Information:
This work was supported by grant no. BT/PR/5799/MED/14/698/2005 from the Department of Biotechnology to A.B. S.D. and M. K.M. is a recipient of Senior Research Fellowship from University Grants Commission, Government of India. The authors thank Mr. Kanhaiya Lal Kumawat for excellent technical assistance. We thank Prof. Vijayalakshmi Ravindranath, Director NBRC for her continuous support and encouragement.
PY - 2008/3
Y1 - 2008/3
N2 - IL-1β and IL-18 are members of the IL-1 family of ligands, and their receptors are members of the IL-1 receptor family. Although several biological properties overlap for these cytokines, differences exist. In order to assess functional importance of these two cytokines in viral encephalitis, we have exploited an experimental model of Japanese Encephalitis (JE) and subsequent in vitro cell culture system. We report for the first time that in Japanese Encephalitis, microglia and astrocytes both produce IL-18 and IL-1β. In vitro, these two cytokines differentially activate microglia and astrocyte, and also alter the by stander neuronal survival following treatment with these two cytokines.
AB - IL-1β and IL-18 are members of the IL-1 family of ligands, and their receptors are members of the IL-1 receptor family. Although several biological properties overlap for these cytokines, differences exist. In order to assess functional importance of these two cytokines in viral encephalitis, we have exploited an experimental model of Japanese Encephalitis (JE) and subsequent in vitro cell culture system. We report for the first time that in Japanese Encephalitis, microglia and astrocytes both produce IL-18 and IL-1β. In vitro, these two cytokines differentially activate microglia and astrocyte, and also alter the by stander neuronal survival following treatment with these two cytokines.
KW - Astrocytes
KW - IL-18
KW - IL-1β
KW - Japanese Encephalitis Virus
KW - Microglia
KW - Neuron
UR - http://www.scopus.com/inward/record.url?scp=41949087849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41949087849&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2008.01.009
DO - 10.1016/j.jneuroim.2008.01.009
M3 - Article
C2 - 18374991
AN - SCOPUS:41949087849
SN - 0165-5728
VL - 195
SP - 60
EP - 72
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
IS - 1-2
ER -