TY - JOUR
T1 - J774 murine macrophage-like cell interactions with Cryptococcus neoformans in the presence and absence of opsonins
AU - Mukherjee, Sushovita
AU - Feldmesser, Marta
AU - Casadevall, Arturo
N1 - Funding Information:
Received 11 October 1995; revised 28 December 1995. Presented in part: Clinical Research Meeting (Association of American Physicians, American Society for Clinical Investigation, and American Federation for Clinical Research), San Diego, May 1995. Grant support: James S. McDonnell Scholar Award (to A.C.); National Institutes of Health (AI-33774, AI-13342; CA-09173 and AI-077183 [to S.M. and M.F.]). Reprints or correspondence: Dr. A. Casadevall, Dept. of Medicine, 1300 Morris Park Ave., Room U1219, Bronx, NY 10461.
PY - 1996
Y1 - 1996
N2 - The interaction of Cryptococcus neoformans with the murine macrophage- like cell line J774.16 was studied in the presence and absence of monoclonal antibodies (MAbs) to the capsular polysaccharide glucuronoxylomannan (GXM). In the absence of MAb 2H1 to GXM, coincubation of J774.16 cells with C. neoformans reduced fungal colony-forming units in only 26.6% of 21 independent experiments. In the presence of MAb 2H1, coincubation of J774.16 cells with C. neoformans reduced fungal colony-forming units in >95% of experiments. Comparison of the relative efficacy of two IgG1 MAbs revealed that the higher affinity MAb was more effective at low concentrations. Antibody-mediated reductions of C. neoformans colony-forming units by J774.16 cells occurred despite inhibition of nitric oxide synthase, addition of reactive oxygen intermediate scavengers, or the use of a superoxide-deficient J774 mutant line.
AB - The interaction of Cryptococcus neoformans with the murine macrophage- like cell line J774.16 was studied in the presence and absence of monoclonal antibodies (MAbs) to the capsular polysaccharide glucuronoxylomannan (GXM). In the absence of MAb 2H1 to GXM, coincubation of J774.16 cells with C. neoformans reduced fungal colony-forming units in only 26.6% of 21 independent experiments. In the presence of MAb 2H1, coincubation of J774.16 cells with C. neoformans reduced fungal colony-forming units in >95% of experiments. Comparison of the relative efficacy of two IgG1 MAbs revealed that the higher affinity MAb was more effective at low concentrations. Antibody-mediated reductions of C. neoformans colony-forming units by J774.16 cells occurred despite inhibition of nitric oxide synthase, addition of reactive oxygen intermediate scavengers, or the use of a superoxide-deficient J774 mutant line.
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U2 - 10.1093/infdis/173.5.1222
DO - 10.1093/infdis/173.5.1222
M3 - Article
C2 - 8627076
AN - SCOPUS:0029893009
SN - 0022-1899
VL - 173
SP - 1222
EP - 1231
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -