IVICAT for the masses

An improved technique for permethylation of peptides

James G. Farmar, Heinz Nika, Fa Yun Che, Louis M. Weiss, Ruth Hogue Angeletti

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To determine the levels of post-translational modifications, we needed a quantitative technique that would allow comparison of the amounts of acetylated versus mono-, di-, and tri-methylated lysines in histones. One method, IVICAT, generates trimethyl-amines and could be used, but is technically challenging. We have modified this technique to be used with standard laboratory equipment so that this chemistry is accessible to most proteomics laboratories.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalJournal of Biomolecular Techniques
Volume20
Issue number5
StatePublished - 2009

Fingerprint

Peptides
Post Translational Protein Processing
Proteomics
Histones
Lysine
Amines
Equipment and Supplies

Keywords

  • Amino groups
  • Quantitation

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Farmar, J. G., Nika, H., Che, F. Y., Weiss, L. M., & Angeletti, R. H. (2009). IVICAT for the masses: An improved technique for permethylation of peptides. Journal of Biomolecular Techniques, 20(5), 285-292.

IVICAT for the masses : An improved technique for permethylation of peptides. / Farmar, James G.; Nika, Heinz; Che, Fa Yun; Weiss, Louis M.; Angeletti, Ruth Hogue.

In: Journal of Biomolecular Techniques, Vol. 20, No. 5, 2009, p. 285-292.

Research output: Contribution to journalArticle

Farmar, JG, Nika, H, Che, FY, Weiss, LM & Angeletti, RH 2009, 'IVICAT for the masses: An improved technique for permethylation of peptides', Journal of Biomolecular Techniques, vol. 20, no. 5, pp. 285-292.
Farmar, James G. ; Nika, Heinz ; Che, Fa Yun ; Weiss, Louis M. ; Angeletti, Ruth Hogue. / IVICAT for the masses : An improved technique for permethylation of peptides. In: Journal of Biomolecular Techniques. 2009 ; Vol. 20, No. 5. pp. 285-292.
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