IVICAT for the masses: An improved technique for permethylation of peptides

James G. Farmar, Heinz Nika, Fa Yun Che, Louis Weiss, Ruth Hogue Angeletti

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

To determine the levels of post-translational modifications, we needed a quantitative technique that would allow comparison of the amounts of acetylated versus mono-, di-, and tri-methylated lysines in histones. One method, IVICAT, generates trimethyl-amines and could be used, but is technically challenging. We have modified this technique to be used with standard laboratory equipment so that this chemistry is accessible to most proteomics laboratories.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalJournal of Biomolecular Techniques
Volume20
Issue number5
StatePublished - 2009

Keywords

  • Amino groups
  • Quantitation

ASJC Scopus subject areas

  • Molecular Biology

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