IVICAT for the masses: An improved technique for permethylation of peptides

James G. Farmar; Heinz Nika; Fa Yun Che; Louis Weiss; Ruth Hogue Angeletti

IVICAT for the masses: An improved technique for permethylation of peptides

James G. Farmar, Heinz Nika, Fa Yun Che, Louis Weiss, Ruth Hogue Angeletti

Research output: Contribution to journal › Article › peer-review

Abstract

To determine the levels of post-translational modifications, we needed a quantitative technique that would allow comparison of the amounts of acetylated versus mono-, di-, and tri-methylated lysines in histones. One method, IVICAT, generates trimethyl-amines and could be used, but is technically challenging. We have modified this technique to be used with standard laboratory equipment so that this chemistry is accessible to most proteomics laboratories.

Original language	English (US)
Pages (from-to)	285-292
Number of pages	8
Journal	Journal of Biomolecular Techniques
Volume	20
Issue number	5
State	Published - 2009

Keywords

Amino groups
Quantitation

ASJC Scopus subject areas

Molecular Biology

Cite this

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abstract = "To determine the levels of post-translational modifications, we needed a quantitative technique that would allow comparison of the amounts of acetylated versus mono-, di-, and tri-methylated lysines in histones. One method, IVICAT, generates trimethyl-amines and could be used, but is technically challenging. We have modified this technique to be used with standard laboratory equipment so that this chemistry is accessible to most proteomics laboratories.",

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AU - Farmar, James G.

AU - Nika, Heinz

AU - Che, Fa Yun

AU - Weiss, Louis

AU - Angeletti, Ruth Hogue

PY - 2009

Y1 - 2009

N2 - To determine the levels of post-translational modifications, we needed a quantitative technique that would allow comparison of the amounts of acetylated versus mono-, di-, and tri-methylated lysines in histones. One method, IVICAT, generates trimethyl-amines and could be used, but is technically challenging. We have modified this technique to be used with standard laboratory equipment so that this chemistry is accessible to most proteomics laboratories.

AB - To determine the levels of post-translational modifications, we needed a quantitative technique that would allow comparison of the amounts of acetylated versus mono-, di-, and tri-methylated lysines in histones. One method, IVICAT, generates trimethyl-amines and could be used, but is technically challenging. We have modified this technique to be used with standard laboratory equipment so that this chemistry is accessible to most proteomics laboratories.

KW - Amino groups

KW - Quantitation

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JO - Journal of Biomolecular Techniques

JF - Journal of Biomolecular Techniques

IS - 5

ER -

IVICAT for the masses: An improved technique for permethylation of peptides

Abstract

Keywords

ASJC Scopus subject areas

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