Isotope-edited FTIR of alkaline phosphatase resolves paradoxical ligand binding properties and suggests a role for ground-state destabilization

Engineering & Materials Science

• Phosphatases 100%
• Ground state 89%
• Ligands 79%
• Isotopes 73%
• Fourier transforms 58%
• Infrared radiation 51%
• Proton transfer 24%
• Protons 23%
• Phosphates 22%
• Electrostatics 21%
• Negative ions 17%
• Catalysis 17%
• Nucleophiles 13%
• Coulomb interactions 10%
• Substrates 9%
• Escherichia coli 8%
• Fourier transform infrared spectroscopy 8%
• Esters 7%
• Atoms 6%

Medicine & Life Sciences

• Fourier Analysis 70%
• Isotopes 64%
• Alkaline Phosphatase 53%
• Ligands 42%
• Protons 33%
• Static Electricity 31%
• Phosphates 17%
• Catalysis 15%
• Anions 14%
• Catalytic Domain 11%
• Fourier Transform Infrared Spectroscopy 7%
• Esters 6%
• Serine 6%
• Escherichia coli 4%

Chemical Compounds

• Ground State 47%
• Phosphoryl 40%
• Fourier Transform Infrared Spectroscopy 33%
• Proton 31%
• Phosphate Monoester 18%
• Catalysis 15%
• Oxyanion 14%
• Inorganic Phosphate 14%
• Anion 13%
• Serine 11%
• Proton Transfer 10%
• Nucleophile 10%
• Transition State 9%
• Kd 9%
• Phosphate 7%
• Ester 7%
• Ligand 5%