Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidium parvum

Kami Kim, Lisa Goozé, Carolyn Petersen, Jiri Gut, Richard G. Nelson

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Actin is an ubiquitous and highly conserved microfilament protein which is hypothesized to play a mechanical force-generating role in the unusual gliding motility of sporozoan zoites and in their active penetration of host cells. We have identified and isolated an actin gene from a Cryptosporidium parvum genomic DNA library using a chicken β-actin cDNA as an hybridization probe. The nucleotide sequences of two overlapping recombinant clones were identical and the amino acid sequence deduced from the single open reading frame was 85% identical to the P. falciparum actin I and human γ-actin proteins. The predicted 42 106-Da Cryptosporidium actin contains 376 amino acids and is encoded by a single-copy gene which contains no introns. The nucleic acid coding sequence is 72% biased to the use of A or T in the third position of codons. Chromosome-sized DNA released from intact C. parvum oocysts was resolved by OFAGE into 5 discrete ethidium bromide-staining DNAs ranging in size from 900 to 1400 kb; the cloned C. parvum actin gene hybridized to a single chromosomal DNA of approximately 1200 kb.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume50
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Cryptosporidium parvum
Karyotype
Actins
Genes
DNA
Microfilament Proteins
Cryptosporidium
Ethidium
Oocysts
Genomic Library
Gene Library
Codon
Introns
Nucleic Acids
Open Reading Frames
Amino Acid Sequence
Chickens
Complementary DNA
Clone Cells
Chromosomes

Keywords

  • Actin
  • Apicomplexa
  • Cryptosporidium parvum
  • Gliding motility
  • Molecular karyotype
  • Pulsed field gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidium parvum. / Kim, Kami; Goozé, Lisa; Petersen, Carolyn; Gut, Jiri; Nelson, Richard G.

In: Molecular and Biochemical Parasitology, Vol. 50, No. 1, 1992, p. 105-113.

Research output: Contribution to journalArticle

Kim, Kami ; Goozé, Lisa ; Petersen, Carolyn ; Gut, Jiri ; Nelson, Richard G. / Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidium parvum. In: Molecular and Biochemical Parasitology. 1992 ; Vol. 50, No. 1. pp. 105-113.
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