Isolation of Synthetic Antibodies Against BCL-2-Associated X Protein (BAX)

Zhou Dai, Jonathan R. Lai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The BCL-2 protein family plays central roles in the mitochondrial pathway of cell apoptosis. The BCL-2-Associated X protein (BAX), along with other proapoptotic proteins, induces cell death in response to a variety of stress stimuli. Upon receipt of killing signals, cytosolic BAX is activated and translocates to mitochondria where it causes mitochondrial outer membrane permeabilization (MOMP) and initials a series of cellular events that eventually lead to cell destruction. Despite recent progress toward understanding the structure, function, and activation mechanism of BAX, detailed information about how cytosolic BAX can be inhibited is still limited. Here we describe a method of selecting synthetic antibody fragments (Fabs) against BAX using phage display. Synthetic antibodies discovered from the selection have been used as structural probes to gain novel mechanistic details on BAX inhibition. This synthetic antibody selection method could be potentially applied to other BCL-2 proteins.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages351-357
Number of pages7
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1877
ISSN (Print)1064-3745

Keywords

  • Antibody selection
  • Apoptosis
  • BAX
  • Fab
  • Phage display
  • Synthetic antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Dai, Z., & Lai, J. R. (2019). Isolation of Synthetic Antibodies Against BCL-2-Associated X Protein (BAX). In Methods in Molecular Biology (pp. 351-357). (Methods in Molecular Biology; Vol. 1877). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8861-7_21