Isolation of multiple normal and functionally defective forms of uridine diphosphate-glucuronosyltransferase from inbred Gunn rats

N. Roy Chowdhury, F. Gross, A. D. Moscioni, M. Kram, I. M. Arias, Jayanta Roy-Chowdhury

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Abstract

Gunn rats are a mutant strain of Wistar rats that have unconjugated hyperbilirubinemia due to absence of hepatic uridine diphosphate-glucuronosyltransferase (UDPGT; EC.2.4.1.17) activity toward bilirubin. We isolated five UDPGT isoforms from solubilized microsomal fractions from liver of inbred Wistar (RHA) rats and congeneic Gunn rats. UDPGT isoform V (elution pH 7.5) from Wistar (RHA) rats is active toward bilirubin and 4'-hydroxydimethylaminoazobenzene. The corresponding isoform from Gunn rat liver was enzymically inactive but exhibited normal elution pH and mobility on NaDodSO4/polyacrylamide gel electrophoresis (M(r) 53,000), and was recognized by a UDPGT-specific antiserum. UDPGT isoform I (elution pH 8.7) from Wistar (RHA) and Gunn rats was active toward 4-nitrophenol. The isoform from Gunn rat liver had only 10% of normal UDPGT activity, however UDPGT activity increased to normal upon addition of 15 mM diethylnitrosamine in vitro. Isoforms II (elution pH 8.4), III (elution pH 8.0), and IV (elution pH 7.8) from Gunn rats had normal UDPGT activities, except that isoform IV was inactive toward bilirubin.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalJournal of Clinical Investigation
Volume79
Issue number2
StatePublished - 1987
Externally publishedYes

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Gunn Rats
Glucuronosyltransferase
Uridine Diphosphate
Protein Isoforms
Wistar Rats
Bilirubin
Liver
Mutant Strains Rats
Diethylnitrosamine
Hyperbilirubinemia
Immune Sera
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Medicine(all)

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Isolation of multiple normal and functionally defective forms of uridine diphosphate-glucuronosyltransferase from inbred Gunn rats. / Roy Chowdhury, N.; Gross, F.; Moscioni, A. D.; Kram, M.; Arias, I. M.; Roy-Chowdhury, Jayanta.

In: Journal of Clinical Investigation, Vol. 79, No. 2, 1987, p. 327-334.

Research output: Contribution to journalArticle

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T1 - Isolation of multiple normal and functionally defective forms of uridine diphosphate-glucuronosyltransferase from inbred Gunn rats

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AU - Gross, F.

AU - Moscioni, A. D.

AU - Kram, M.

AU - Arias, I. M.

AU - Roy-Chowdhury, Jayanta

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