Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II

Annette Nemeth, Sabine Krause, Diana Blank, Andreas Jenny, Paul Jenö, Ariel Lustig, Elmar Wahle

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

cDNA clones for bovine poly(A) binding protein II (PAB II) were isolated. Their sequence predicts a protein of 32.8 kDa, revising earlier estimates of molecular mass. The protein contains one putative RNA-binding domain of the RNP type, an acidic N-terminal and a basic C-terminal domain. Analyses of authentic PAB II were in good agreement with all predictions from the cDNA sequence except that a number of arginine residues appeared to be post-translationally modified. Poly(A) binding protein II expressed in Escherichia coli was active in poly(A) binding and reconstitution of processive polyadenylation, including poly(A) tail length control. The cDNA clones showed a number of potential PAB II binding sites in the 3′ untranslated sequence. Bovine poly(A)+ RNA contained two mRNAs hybridizing to a PAB II-specific probe. Analysis of a genomic clone revealed six introns in the coding sequence. The revised molecular mass led to a demonstration of PAB II oligomer formation and a reinterpretation of earlier data concerning the protein's binding to poly(A).

Original languageEnglish (US)
Pages (from-to)4034-4041
Number of pages8
JournalNucleic Acids Research
Volume23
Issue number20
DOIs
StatePublished - Oct 25 1995
Externally publishedYes

Fingerprint

Poly(A)-Binding Protein II
CDNA
Clone
Isolation
Genomics
Encoding
Complementary DNA
Clone Cells
Molecular mass
Protein
Proteins
Arginine
Binding sites
Poly A
RNA
Oligomers
Escherichia coli
Messenger RNA
Demonstrations
Poly(A)-Binding Proteins

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Nemeth, A., Krause, S., Blank, D., Jenny, A., Jenö, P., Lustig, A., & Wahle, E. (1995). Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II. Nucleic Acids Research, 23(20), 4034-4041. https://doi.org/10.1093/nar/23.20.4034

Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II. / Nemeth, Annette; Krause, Sabine; Blank, Diana; Jenny, Andreas; Jenö, Paul; Lustig, Ariel; Wahle, Elmar.

In: Nucleic Acids Research, Vol. 23, No. 20, 25.10.1995, p. 4034-4041.

Research output: Contribution to journalArticle

Nemeth, A, Krause, S, Blank, D, Jenny, A, Jenö, P, Lustig, A & Wahle, E 1995, 'Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II', Nucleic Acids Research, vol. 23, no. 20, pp. 4034-4041. https://doi.org/10.1093/nar/23.20.4034
Nemeth, Annette ; Krause, Sabine ; Blank, Diana ; Jenny, Andreas ; Jenö, Paul ; Lustig, Ariel ; Wahle, Elmar. / Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II. In: Nucleic Acids Research. 1995 ; Vol. 23, No. 20. pp. 4034-4041.
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