TY - JOUR
T1 - Isolation, genomic organization, and expression analysis of Men1, the murine homolog of the MEN1 gene
AU - Guru, Siradanahalli C.
AU - Crabtree, Judy S.
AU - Brown, Kevin D.
AU - Dunn, Karen J.
AU - Manickam, Pachiappan
AU - Prasad, Nijaguna B.
AU - Wangsa, Danny
AU - Lee Burns, A.
AU - Spiegel, Allen M.
AU - Marx, Stephen J.
AU - Pavan, William J.
AU - Collins, Francis S.
AU - Chandrasekharappa, Settara C.
PY - 1999
Y1 - 1999
N2 - The mouse homolog of the human MEN1 gene, which is defective in a dominant familial cancer syndrome, multiple endocrine neoplasia type 1 (MEN1), has been identified and characterized. The mouse Men1 transcript contains an open reading frame encoding a protein of 611 amino acids which has 97% identity and 98% similarity to human menin. Sequence of the entire Men1 gene (9.3 kb) was assembled, revealing 10 exons, with exon 1 being non- coding; a polymorphic tetranucleotide repeat was located in the 5'-flanking region. The exon-intron organization and the size of the coding exons 2-9 were well conserved between the human and mouse genes. Fluorescence in situ hybridization localized the Men1 gene to mouse Chromosome (Chr) 19, a region known to be syntenic to human Chr 11q13, the locus for the MEN1 gene. Northern analysis indicated two messages-2.7 kb and 3.1 kb-expressed in all stages of the embryo analyzed and in all eight adult tissues tested. The larger transcript differs from the smaller by the inclusion of an unspliced intron 1. Whole-mount in situ hybridization of 10.5-day and 11.5-day embryos showed ubiquitous expression of Men1 RNA. Western analysis with antibodies raised against a conserved C-terminal peptide identified an approximately 67- kDa protein in the lysates of adult mouse brain, kidney, liver, pancreas, and spleen tissues, consistent with the size of human menin. The levels of mouse menin do not appear to fluctuate during the cell cycle.
AB - The mouse homolog of the human MEN1 gene, which is defective in a dominant familial cancer syndrome, multiple endocrine neoplasia type 1 (MEN1), has been identified and characterized. The mouse Men1 transcript contains an open reading frame encoding a protein of 611 amino acids which has 97% identity and 98% similarity to human menin. Sequence of the entire Men1 gene (9.3 kb) was assembled, revealing 10 exons, with exon 1 being non- coding; a polymorphic tetranucleotide repeat was located in the 5'-flanking region. The exon-intron organization and the size of the coding exons 2-9 were well conserved between the human and mouse genes. Fluorescence in situ hybridization localized the Men1 gene to mouse Chromosome (Chr) 19, a region known to be syntenic to human Chr 11q13, the locus for the MEN1 gene. Northern analysis indicated two messages-2.7 kb and 3.1 kb-expressed in all stages of the embryo analyzed and in all eight adult tissues tested. The larger transcript differs from the smaller by the inclusion of an unspliced intron 1. Whole-mount in situ hybridization of 10.5-day and 11.5-day embryos showed ubiquitous expression of Men1 RNA. Western analysis with antibodies raised against a conserved C-terminal peptide identified an approximately 67- kDa protein in the lysates of adult mouse brain, kidney, liver, pancreas, and spleen tissues, consistent with the size of human menin. The levels of mouse menin do not appear to fluctuate during the cell cycle.
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U2 - 10.1007/s003359901051
DO - 10.1007/s003359901051
M3 - Article
C2 - 10341092
AN - SCOPUS:0032769061
SN - 0938-8990
VL - 10
SP - 592
EP - 596
JO - Mammalian Genome
JF - Mammalian Genome
IS - 6
ER -