Isolation, characterization and sequencing of the chicken apolipoprotein-AI-encoding gene

Nisan Bhattacharyya, Ranjan Chattapadhyay, Allen Hirsch, Debendranath Banerjee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The chicken ApoAI gene has been isolated and characterized. This gene contains three introns: the first is situated after nucleotide (nt) 41 in the 5'-untranslated region of the gene, the second interrupts the codon specifying the Gly-10 of the prepeptide, and the third disrupts the codon for Asp42 in the mature ApoAl protein. The chicken ApoAI gene has 62.6% sequence similarity with the human and 65.0% similarity with the rat gene. Intron-exon organization of the chicken gene is similar to that of human and rat ApoAI genes. Two different transcriptional start points (tsp) only 2 nt apart from each other, have been obtained for the chicken ApoAI gene. The 5'-flanking sequence of the gene contains TAAATA (TATA-like box) and CCACAT (CCAAT-like box) sequences, which are located 21 bp and 96 bp upstream from the tsp respectively. This gene's sequence and structural organization provide a basis for future studies of the regulation of chicken ApoAI gene expression.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalGene
Volume104
Issue number2
DOIs
StatePublished - Aug 15 1991

Keywords

  • Genomic organization
  • exon
  • intron
  • nucleotide sequence comparison
  • promoter
  • recombinant DNA
  • transcription start points

ASJC Scopus subject areas

  • Genetics

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