TY - JOUR
T1 - Isolation, characterization and sequencing of the chicken apolipoprotein-AI-encoding gene
AU - Bhattacharyya, Nisan
AU - Chattapadhyay, Ranjan
AU - Hirsch, Allen
AU - Banerjee, Debendranath
N1 - Funding Information:
We thank Tellervo Huima and Valerie A. Williams for their help with figure preparation and photography, Dr. Colvin Redman for his constant encouragement. support and many helpful suggestionst hroughout the course of this study, the Microchemistry Laboratory for synthesizing oli-gos and Dr. S. Ferrari (Modena, Italy) for cDNA clones. This work was supported by National Institutes of Health grant HL 36699.
PY - 1991/8/15
Y1 - 1991/8/15
N2 - The chicken ApoAI gene has been isolated and characterized. This gene contains three introns: the first is situated after nucleotide (nt) 41 in the 5'-untranslated region of the gene, the second interrupts the codon specifying the Gly-10 of the prepeptide, and the third disrupts the codon for Asp42 in the mature ApoAl protein. The chicken ApoAI gene has 62.6% sequence similarity with the human and 65.0% similarity with the rat gene. Intron-exon organization of the chicken gene is similar to that of human and rat ApoAI genes. Two different transcriptional start points (tsp) only 2 nt apart from each other, have been obtained for the chicken ApoAI gene. The 5'-flanking sequence of the gene contains TAAATA (TATA-like box) and CCACAT (CCAAT-like box) sequences, which are located 21 bp and 96 bp upstream from the tsp respectively. This gene's sequence and structural organization provide a basis for future studies of the regulation of chicken ApoAI gene expression.
AB - The chicken ApoAI gene has been isolated and characterized. This gene contains three introns: the first is situated after nucleotide (nt) 41 in the 5'-untranslated region of the gene, the second interrupts the codon specifying the Gly-10 of the prepeptide, and the third disrupts the codon for Asp42 in the mature ApoAl protein. The chicken ApoAI gene has 62.6% sequence similarity with the human and 65.0% similarity with the rat gene. Intron-exon organization of the chicken gene is similar to that of human and rat ApoAI genes. Two different transcriptional start points (tsp) only 2 nt apart from each other, have been obtained for the chicken ApoAI gene. The 5'-flanking sequence of the gene contains TAAATA (TATA-like box) and CCACAT (CCAAT-like box) sequences, which are located 21 bp and 96 bp upstream from the tsp respectively. This gene's sequence and structural organization provide a basis for future studies of the regulation of chicken ApoAI gene expression.
KW - Genomic organization
KW - exon
KW - intron
KW - nucleotide sequence comparison
KW - promoter
KW - recombinant DNA
KW - transcription start points
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U2 - 10.1016/0378-1119(91)90246-8
DO - 10.1016/0378-1119(91)90246-8
M3 - Article
C2 - 1916289
AN - SCOPUS:0025944568
SN - 0378-1119
VL - 104
SP - 163
EP - 168
JO - Gene
JF - Gene
IS - 2
ER -