Isolation and partial characterization of lectin-resistant F9 cells

Petr Dráber, Pamela Stanley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cytotoxic plant lectins have been used for the single-step selection of mouse embryonal carcinoma cell mutants with altered expression of surface glycoconjugates. Following mutagenesis, several F9 and OTF9-63 cell lines resistant to the lectins from Triticum vulgaris or Ricinus communis were obtained. At least five distinct lectin-resistant (LecR) phenotypes have been identified on the basis of their relative sensitivities to four different plant lectins and their altered lectin-binding properties. None of the mutant types exhibits a significant change in the ability to bind a monoclonal antibody against the stage-specific embryonic antigen, SSEA-1. All of the mutants form aggregates when cultured in bacteriological petri dishes and appear to differentiate into endoderm-like cells following exposure to retinoic acid. However, two of the LecR cell lines exhibit an altered morphology when grown on a plastic substratum.

Original languageEnglish (US)
Pages (from-to)445-454
Number of pages10
JournalSomatic Cell and Molecular Genetics
Volume10
Issue number5
DOIs
StatePublished - Sep 1984

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Lectins
CD15 Antigens
Plant Lectins
Ricinus
Embryonal Carcinoma Stem Cells
Cell Line
Endoderm
Glycoconjugates
Tretinoin
Mutagenesis
Plastics
Triticum
Monoclonal Antibodies
Phenotype

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Isolation and partial characterization of lectin-resistant F9 cells. / Dráber, Petr; Stanley, Pamela.

In: Somatic Cell and Molecular Genetics, Vol. 10, No. 5, 09.1984, p. 445-454.

Research output: Contribution to journalArticle

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