Isolation and initial characterization of multiple species of T-lymphocyte subset cDNA clones

B. S. Kwon, G. S. Kim, M. B. Prystowsky, D. W. Lancki, D. E. Sabath, J. L. Pan, S. M. Weissman

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

A modified differential screening procedure was applied to analyze cDNA libraries of cloned helper T lymphocytes (Th) and cytolytic T lymphocytes (CTL). Negative and positive differential screening and RNA blot analysis were used to identify cDNA clones that were expressed preferentially in Th or CTL. Seven clones corresponded to previously described T-cell genes, and 16 additional types of cDNA clones were isolated, 9 from Th and 7 from CTL. Of these, 3 were expressed in both Th and CTL, 7 were expressed in only Th, and 6 only in CTL. These clones were analyzed for induction after stimulation by interleukin 2 or Con A or after stimulation of the T-cell antigen receptor (TCR). Three different patterns of expression were seen: (i) induction only by Con A, (ii) induction by Con A and interleukin 2, and (iii) induction by Con A and TCR stimulation. The approach is potentially useful for analyzing paths of T-cell differentiation and detecting cDNA clones encoding unrecognized cytokines.

Original languageEnglish (US)
Pages (from-to)2896-2900
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number9
DOIs
StatePublished - Jun 25 1987
Externally publishedYes

ASJC Scopus subject areas

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