Isolation and identification of a novel cDNA that encodes human yrdC protein

Jinzhong Chen, Chaoneng Ji, Shaohua Gu, Enpeng Zhao, Jianliang Dai, Lu Huang, Ji Qian, Kang Ying, Yi Xie, Yumin Mao

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the course of detecting the interaction protein of RBBP10 by yeast two-hybridization, we isolated a novel cDNA that encodes a putative human protein with yrdC domain. It is named human yrdC protein. Because the cDNA contains an open reading fragment (ORF) without a 5′ in- frame stop codon, 5′ RACE and 3′ RACE were proceeded to produce the full-length cDNA. An 1825 bp cDNA was isolated from human placenta, which encodes a putative protein of 279 amino acids. The protein contains a sua5-yciO-yrdC domain. Blast analysis against the human genome database of Genbank revealed that the gene contains five exons, and assigned the gene to human chromosome 1p34.2. A transcript about 2.5 kb is ubiquitously expressed in human tissues. The gene is highly conserved during evolution.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalJournal of Human Genetics
Volume48
Issue number4
DOIs
StatePublished - 2003
Externally publishedYes

Fingerprint

Complementary DNA
Proteins
Genes
Fungal Proteins
Terminator Codon
Nucleic Acid Databases
Human Chromosomes
Human Genome
Placenta
Reading
Exons
Databases
Amino Acids

Keywords

  • Expression
  • Human yrdC
  • RACE
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Chen, J., Ji, C., Gu, S., Zhao, E., Dai, J., Huang, L., ... Mao, Y. (2003). Isolation and identification of a novel cDNA that encodes human yrdC protein. Journal of Human Genetics, 48(4), 164-169. https://doi.org/10.1007/s10038-002-0001-3

Isolation and identification of a novel cDNA that encodes human yrdC protein. / Chen, Jinzhong; Ji, Chaoneng; Gu, Shaohua; Zhao, Enpeng; Dai, Jianliang; Huang, Lu; Qian, Ji; Ying, Kang; Xie, Yi; Mao, Yumin.

In: Journal of Human Genetics, Vol. 48, No. 4, 2003, p. 164-169.

Research output: Contribution to journalArticle

Chen, J, Ji, C, Gu, S, Zhao, E, Dai, J, Huang, L, Qian, J, Ying, K, Xie, Y & Mao, Y 2003, 'Isolation and identification of a novel cDNA that encodes human yrdC protein', Journal of Human Genetics, vol. 48, no. 4, pp. 164-169. https://doi.org/10.1007/s10038-002-0001-3
Chen, Jinzhong ; Ji, Chaoneng ; Gu, Shaohua ; Zhao, Enpeng ; Dai, Jianliang ; Huang, Lu ; Qian, Ji ; Ying, Kang ; Xie, Yi ; Mao, Yumin. / Isolation and identification of a novel cDNA that encodes human yrdC protein. In: Journal of Human Genetics. 2003 ; Vol. 48, No. 4. pp. 164-169.
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AU - Ji, Chaoneng

AU - Gu, Shaohua

AU - Zhao, Enpeng

AU - Dai, Jianliang

AU - Huang, Lu

AU - Qian, Ji

AU - Ying, Kang

AU - Xie, Yi

AU - Mao, Yumin

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