Isolation and identification of a novel cDNA that encodes human yrdC protein

Jinzhong Chen, Chaoneng Ji, Shaohua Gu, Enpeng Zhao, Jianliang Dai, Lu Huang, Ji Qian, Kang Ying, Yi Xie, Yumin Mao

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

In the course of detecting the interaction protein of RBBP10 by yeast two-hybridization, we isolated a novel cDNA that encodes a putative human protein with yrdC domain. It is named human yrdC protein. Because the cDNA contains an open reading fragment (ORF) without a 5′ in- frame stop codon, 5′ RACE and 3′ RACE were proceeded to produce the full-length cDNA. An 1825 bp cDNA was isolated from human placenta, which encodes a putative protein of 279 amino acids. The protein contains a sua5-yciO-yrdC domain. Blast analysis against the human genome database of Genbank revealed that the gene contains five exons, and assigned the gene to human chromosome 1p34.2. A transcript about 2.5 kb is ubiquitously expressed in human tissues. The gene is highly conserved during evolution.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalJournal of Human Genetics
Volume48
Issue number4
DOIs
StatePublished - Jun 2 2003

Keywords

  • Expression
  • Human yrdC
  • RACE
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Chen, J., Ji, C., Gu, S., Zhao, E., Dai, J., Huang, L., Qian, J., Ying, K., Xie, Y., & Mao, Y. (2003). Isolation and identification of a novel cDNA that encodes human yrdC protein. Journal of Human Genetics, 48(4), 164-169. https://doi.org/10.1007/s10038-002-0001-3