Isolation and characterization of two replication-dependent mouse H1 histone genes

Yonghe Dong, Allen M. Sirotkin, Yih Sheng Yang, David T. Brown, Donald B. Sittman, Arthur I. Skoultchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Mice contain at least seven nonallelic forms of the H1 histones, including the somatic variants H1a-e and less closely related variants H1° and H1t. The mouse H1° and H1c (H1var.1) genes were isolated and characterized previously. We have now isolated, sequenced and studied the expression properties of two additional mouse H1 genes, termed H1var.2 and H1var.3. Extensive amino acid and nucleotide sequence comparisons were made between the two genes and other mammalian H1 histone genes. A high degree of nucleotide sequence identity was seen between the H1var.2, rat H1d and human H1b genes, even well beyond the coding region, indicating that these genes are likely homologues. Unlike the previously characterized mouse H1var.l gene which produces both nonpolyadenylated and polyadenylated mRNAs, the H1var.2 and H1var.3 genes produce only typical, replication dependent, nonpolyadenylated mRNAs.

Original languageEnglish (US)
Pages (from-to)1421-1428
Number of pages8
JournalNucleic Acids Research
Volume22
Issue number8
StatePublished - Apr 25 1994

Fingerprint

Histones
Replication
Isolation
Mouse
Genes
Gene
Dependent
Nucleotides
Messenger RNA
Property of expression
Sequence Comparison
Amino Acids
Amino acids
Rats
Amino Acid Sequence
Coding
Likely

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Dong, Y., Sirotkin, A. M., Yang, Y. S., Brown, D. T., Sittman, D. B., & Skoultchi, A. I. (1994). Isolation and characterization of two replication-dependent mouse H1 histone genes. Nucleic Acids Research, 22(8), 1421-1428.

Isolation and characterization of two replication-dependent mouse H1 histone genes. / Dong, Yonghe; Sirotkin, Allen M.; Yang, Yih Sheng; Brown, David T.; Sittman, Donald B.; Skoultchi, Arthur I.

In: Nucleic Acids Research, Vol. 22, No. 8, 25.04.1994, p. 1421-1428.

Research output: Contribution to journalArticle

Dong, Y, Sirotkin, AM, Yang, YS, Brown, DT, Sittman, DB & Skoultchi, AI 1994, 'Isolation and characterization of two replication-dependent mouse H1 histone genes', Nucleic Acids Research, vol. 22, no. 8, pp. 1421-1428.
Dong Y, Sirotkin AM, Yang YS, Brown DT, Sittman DB, Skoultchi AI. Isolation and characterization of two replication-dependent mouse H1 histone genes. Nucleic Acids Research. 1994 Apr 25;22(8):1421-1428.
Dong, Yonghe ; Sirotkin, Allen M. ; Yang, Yih Sheng ; Brown, David T. ; Sittman, Donald B. ; Skoultchi, Arthur I. / Isolation and characterization of two replication-dependent mouse H1 histone genes. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 8. pp. 1421-1428.
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