TY - JOUR
T1 - Isolation and characterization of two replication-dependent mouse H1 histone genes
AU - Dong, Yonghe
AU - Sirotkin, Allen M.
AU - Yang, Yih sheng
AU - Brown, David T.
AU - Sittman, Donald B.
AU - Skoultchi, Arthur I.
N1 - Funding Information:
This work was supported by National Institutes of Health Grant CA 16368 to A.I.S., National Science Foundation Grant DMB-8818958 and a grant from the Mississippi Affiliate of the American Heart Association to D.B.S. and by an Albert Einstein College of Medicine Core Grant (NIH 2 P30 CA13330) A.M.S. was supported by NIH Training Grant GM 07491.
PY - 1994/4/25
Y1 - 1994/4/25
N2 - Mice contain at least seven nonallelic forms of the H1 hlstones, including the somatic variants H1a-e and less closely related variants H1° and Hit. The mouse H1° and H1c (H1var.1) genes were isolated and characterized previously. We have now Isolated, sequenced and studied the expression properties of two additional mouse H1 genes, termed H1var.2 and H1var.3. Extensive amino acid and nucleotlde sequence comparisons were made between the two genes and other mammalian H1 histone genes. A high degree of nucleotlde sequence Identity was seen between the H1var.2, rat H1d and human H1b genes, even well beyond the coding region, indicating that these genes are likely homologues. Unlike the previously characterized mouse H1var.1 gene which produces both nonpolyadenylated and polyadenylated mRNAs, the H1var.2 and H1var.3 genes produce only typical, replication dependent, nonpolyadenylated mRNAs.
AB - Mice contain at least seven nonallelic forms of the H1 hlstones, including the somatic variants H1a-e and less closely related variants H1° and Hit. The mouse H1° and H1c (H1var.1) genes were isolated and characterized previously. We have now Isolated, sequenced and studied the expression properties of two additional mouse H1 genes, termed H1var.2 and H1var.3. Extensive amino acid and nucleotlde sequence comparisons were made between the two genes and other mammalian H1 histone genes. A high degree of nucleotlde sequence Identity was seen between the H1var.2, rat H1d and human H1b genes, even well beyond the coding region, indicating that these genes are likely homologues. Unlike the previously characterized mouse H1var.1 gene which produces both nonpolyadenylated and polyadenylated mRNAs, the H1var.2 and H1var.3 genes produce only typical, replication dependent, nonpolyadenylated mRNAs.
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U2 - 10.1093/nar/22.8.1421
DO - 10.1093/nar/22.8.1421
M3 - Article
C2 - 8190634
AN - SCOPUS:0028308325
SN - 0305-1048
VL - 22
SP - 1421
EP - 1428
JO - Nucleic acids research
JF - Nucleic acids research
IS - 8
ER -