Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice

Kajohnkiart Janebodin, Orapin V. Horst, Nicholas Ieronimakis, Gayathri Balasundaram, Kanit Reesukumal, Busadee Pratumvinit, Morayma Reyes Gil

Research output: Contribution to journalArticle

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Abstract

Dental pulp stem cells (DPSCs) are shown to reside within the tooth and play an important role in dentin regeneration. DPSCs were first isolated and characterized from human teeth and most studies have focused on using this adult stem cell for clinical applications. However, mouse DPSCs have not been well characterized and their origin(s) have not yet been elucidated. Herein we examined if murine DPSCs are neural crest derived and determined their in vitro and in vivo capacity. DPSCs from neonatal murine tooth pulp expressed embryonic stem cell and neural crest related genes, but lacked expression of mesodermal genes. Cells isolated from the Wnt1-Cre/R26R-LacZ model, a reporter of neural crest-derived tissues, indicated that DPSCs were Wnt1-marked and therefore of neural crest origin. Clonal DPSCs showed multi-differentiation in neural crest lineage for odontoblasts, chondrocytes, adipocytes, neurons, and smooth muscles. Following in vivo subcutaneous transplantation with hydroxyapatite/tricalcium phosphate, based on tissue/cell morphology and specific antibody staining, the clones differentiated into odontoblast-like cells and produced dentin-like structure. Conversely, bone marrow stromal cells (BMSCs) gave rise to osteoblast-like cells and generated bone-like structure. Interestingly, the capillary distribution in the DPSC transplants showed close proximity to odontoblasts whereas in the BMSC transplants bone condensations were distant to capillaries resembling dentinogenesis in the former vs. osteogenesis in the latter. Thus we demonstrate the existence of neural crest-derived DPSCs with differentiation capacity into cranial mesenchymal tissues and other neural crest-derived tissues. In turn, DPSCs hold promise as a source for regenerating cranial mesenchyme and other neural crest derived tissues.

Original languageEnglish (US)
Article numbere27526
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 8 2011
Externally publishedYes

Fingerprint

tooth pulp
Dental Pulp
neural crest
Neural Crest
Stem cells
Pulp
stem cells
neonates
Stem Cells
Odontoblasts
teeth
Tissue
Bone
Tooth
Dentin
stromal cells
Transplants
bone marrow cells
Mesenchymal Stromal Cells
Dentinogenesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Janebodin, K., Horst, O. V., Ieronimakis, N., Balasundaram, G., Reesukumal, K., Pratumvinit, B., & Reyes Gil, M. (2011). Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice. PLoS One, 6(11), [e27526]. https://doi.org/10.1371/journal.pone.0027526

Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice. / Janebodin, Kajohnkiart; Horst, Orapin V.; Ieronimakis, Nicholas; Balasundaram, Gayathri; Reesukumal, Kanit; Pratumvinit, Busadee; Reyes Gil, Morayma.

In: PLoS One, Vol. 6, No. 11, e27526, 08.11.2011.

Research output: Contribution to journalArticle

Janebodin, K, Horst, OV, Ieronimakis, N, Balasundaram, G, Reesukumal, K, Pratumvinit, B & Reyes Gil, M 2011, 'Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice', PLoS One, vol. 6, no. 11, e27526. https://doi.org/10.1371/journal.pone.0027526
Janebodin K, Horst OV, Ieronimakis N, Balasundaram G, Reesukumal K, Pratumvinit B et al. Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice. PLoS One. 2011 Nov 8;6(11). e27526. https://doi.org/10.1371/journal.pone.0027526
Janebodin, Kajohnkiart ; Horst, Orapin V. ; Ieronimakis, Nicholas ; Balasundaram, Gayathri ; Reesukumal, Kanit ; Pratumvinit, Busadee ; Reyes Gil, Morayma. / Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice. In: PLoS One. 2011 ; Vol. 6, No. 11.
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