Isolation and characterization of chromaffin granules from a pheochromocytoma (PC 12) cell line

L. Giorgio Roda; Judith A. Nolan; Seung U. Kim; Ruth A. Hogue-Angeletti

doi:10.1016/0014-4827(80)90392-4

Isolation and characterization of chromaffin granules from a pheochromocytoma (PC 12) cell line

L. Giorgio Roda, Judith A. Nolan, Seung U. Kim, Ruth A. Hogue-Angeletti

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Abstract

Chromaffin granules have been isolated from the pheochromocytoma clonal cell line, PC 12, The PC 12 cells, preloaded with radioactive catecholamines, were homogenized in isotonic sucrose and subjected to differential centrifugation and linear or stepwise sucrose gradient fractionation. Antigenic determinants characteristic of bovine chromaffin granules were detected both by immunofluorescence in intact cells and by complement fixation of isolated PC 12 granules. Electrophoretic analysis of the purified PC 12 granules revealed that the major proteins present have molecular weights similar to those of bovine chromaffin granule protein markers. The PC 12 catecholamine storage granules are thus suitable in vitro models for adrenal chromaffin granules.

Original language	English (US)
Pages (from-to)	103-109
Number of pages	7
Journal	Experimental Cell Research
Volume	128
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(80)90392-4
State	Published - 1980
Externally published	Yes

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Cell Biology

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title = "Isolation and characterization of chromaffin granules from a pheochromocytoma (PC 12) cell line",

abstract = "Chromaffin granules have been isolated from the pheochromocytoma clonal cell line, PC 12, The PC 12 cells, preloaded with radioactive catecholamines, were homogenized in isotonic sucrose and subjected to differential centrifugation and linear or stepwise sucrose gradient fractionation. Antigenic determinants characteristic of bovine chromaffin granules were detected both by immunofluorescence in intact cells and by complement fixation of isolated PC 12 granules. Electrophoretic analysis of the purified PC 12 granules revealed that the major proteins present have molecular weights similar to those of bovine chromaffin granule protein markers. The PC 12 catecholamine storage granules are thus suitable in vitro models for adrenal chromaffin granules.",

author = "Roda, {L. Giorgio} and Nolan, {Judith A.} and Kim, {Seung U.} and Hogue-Angeletti, {Ruth A.}",

year = "1980",

doi = "10.1016/0014-4827(80)90392-4",

language = "English (US)",

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journal = "Experimental Cell Research",

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T1 - Isolation and characterization of chromaffin granules from a pheochromocytoma (PC 12) cell line

AU - Roda, L. Giorgio

AU - Nolan, Judith A.

AU - Kim, Seung U.

AU - Hogue-Angeletti, Ruth A.

PY - 1980

Y1 - 1980

N2 - Chromaffin granules have been isolated from the pheochromocytoma clonal cell line, PC 12, The PC 12 cells, preloaded with radioactive catecholamines, were homogenized in isotonic sucrose and subjected to differential centrifugation and linear or stepwise sucrose gradient fractionation. Antigenic determinants characteristic of bovine chromaffin granules were detected both by immunofluorescence in intact cells and by complement fixation of isolated PC 12 granules. Electrophoretic analysis of the purified PC 12 granules revealed that the major proteins present have molecular weights similar to those of bovine chromaffin granule protein markers. The PC 12 catecholamine storage granules are thus suitable in vitro models for adrenal chromaffin granules.

AB - Chromaffin granules have been isolated from the pheochromocytoma clonal cell line, PC 12, The PC 12 cells, preloaded with radioactive catecholamines, were homogenized in isotonic sucrose and subjected to differential centrifugation and linear or stepwise sucrose gradient fractionation. Antigenic determinants characteristic of bovine chromaffin granules were detected both by immunofluorescence in intact cells and by complement fixation of isolated PC 12 granules. Electrophoretic analysis of the purified PC 12 granules revealed that the major proteins present have molecular weights similar to those of bovine chromaffin granule protein markers. The PC 12 catecholamine storage granules are thus suitable in vitro models for adrenal chromaffin granules.

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Isolation and characterization of chromaffin granules from a pheochromocytoma (PC 12) cell line

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