Isolation and characterization of cDNA clones encoding epitopes shared with Alzheimer neurofibrillary tangles

M. Dammerman, M. Goldstein, S. H C Yen, Bridget Shafit-Zagardo

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Abstract

A pool of ten monoclonal antibodies to SDS-insoluble epitopes of Alzheimer neurofibrillary tangles (NFT) was used to screen an adult human brain cDNA expression library. Fourteen clones were isolated, two of which are described. The largest of the clones encodes 80 kD, or approximately 600 amino acids, of microtubule-associated protein 2 (MAP 2). The MAP 2 region encoded by the clone shares at least two epitopes with human tau, another microtubule-associated protein which cross-reacts with NFT. In rat brain mRNA, the MAP 2 cDNA hybridizes to a single transcript of 9.5 kb. In human neuroblastoma mRNA, the MAP 2 cDNA hybridizes, at high stringency, to two transcripts of 9.5 kb and 6 kb. The 6-kb transcript comigrates with the transcript for tau, as detected by a human tau cDNA. The properties of the MAP 2 cDNA suggest that, in humans, MAP 2 and tau have a common domain which may play a role in NFT formation. Another clone isolated with the anti-NFT antibodies shares epitopes, but not nucleic acid homology, with the MAP 2 cDNA. This clone detects a single abundant transcript of 1 kb present in RNA from human nueroblastoma and from several non-neuronal human cell lines. The properties of this cDNA suggest that it encodes a protein other than those previously reported to cross-react with NFT.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalJournal of Neuroscience Research
Volume19
Issue number1
StatePublished - 1988

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Neurofibrillary Tangles
Microtubule-Associated Proteins
Epitopes
Complementary DNA
Clone Cells
Messenger RNA
Brain
Neuroblastoma
Gene Library
Nucleic Acids
Monoclonal Antibodies
RNA
Amino Acids
Cell Line
Antibodies
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Isolation and characterization of cDNA clones encoding epitopes shared with Alzheimer neurofibrillary tangles. / Dammerman, M.; Goldstein, M.; Yen, S. H C; Shafit-Zagardo, Bridget.

In: Journal of Neuroscience Research, Vol. 19, No. 1, 1988, p. 43-51.

Research output: Contribution to journalArticle

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