Isolation and characterization of anti-monosialoganglioside monoclonal antibody 19-9 class-switch variants

Z. Steplewski, G. Spira, M. Blaszczyk, M. D. Lubeck, A. Radbruch, H. Illges, D. Herlyn, K. Rajewsky, Matthew D. Scharff

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Three different monoclonal antibody isotypes, IgG1, IgG2b, and IgG2a, were derived by selection of isotype-switch variants from the CO-19-9 hybridoma. All three antibodies retained their binding specificities and affinities and bound to the same epitope - as defined by the anti-idiotype analysis. Availability of γ1, γ2b, and γ2a Ig heavy chain variants directed against the same epitope on the monosialoganglioside antigen permitted detailed analysis of their Fc fragment receptor (FcR) binding affinities, their cytolytic activities (antibody-dependent, macrophage-mediated cytotoxicity) in vitro, and their tumoricidal activities in vivo. Analysis of the binding of three isotypes to the human FcR expressed by U937 cells induced by γ interferon has shown that only IgG2a proteins bound to high-affinity FcR, but not IgG1 or IgG2b variants. Although all three isotypes were active in the antibody-dependent, macrophage-mediated cytotoxicity assay with murine thioglycolate-elicited macrophages, the IgG2a gave the highest percentage of lysis; similar results were obtained in the same assay with human monocytes as effector cells. In the nude mice experiment, only the IgG2a variant inhibited growth of human colorectal carcinoma, while IgG1 and IgG2b were ineffective. Thus, selection of isotype-switch variants resulted in the conversion of monoclonal antibody from noncytolytic to cytolytic with possible immunotherapeutic application.

Original languageEnglish (US)
Pages (from-to)8653-8657
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number24
StatePublished - 1985
Externally publishedYes

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Immunoglobulin Fc Fragments
Fc Receptors
Immunoglobulin G
Macrophages
Monoclonal Antibodies
Antibodies
Epitopes
Thioglycolates
Immunoglobulin Heavy Chains
U937 Cells
Hybridomas
Carbon Monoxide
Nude Mice
Interferons
Monocytes
Colorectal Neoplasms
Antigens
Growth
sialogangliosides
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Isolation and characterization of anti-monosialoganglioside monoclonal antibody 19-9 class-switch variants. / Steplewski, Z.; Spira, G.; Blaszczyk, M.; Lubeck, M. D.; Radbruch, A.; Illges, H.; Herlyn, D.; Rajewsky, K.; Scharff, Matthew D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 24, 1985, p. 8653-8657.

Research output: Contribution to journalArticle

Steplewski, Z, Spira, G, Blaszczyk, M, Lubeck, MD, Radbruch, A, Illges, H, Herlyn, D, Rajewsky, K & Scharff, MD 1985, 'Isolation and characterization of anti-monosialoganglioside monoclonal antibody 19-9 class-switch variants', Proceedings of the National Academy of Sciences of the United States of America, vol. 82, no. 24, pp. 8653-8657.
Steplewski, Z. ; Spira, G. ; Blaszczyk, M. ; Lubeck, M. D. ; Radbruch, A. ; Illges, H. ; Herlyn, D. ; Rajewsky, K. ; Scharff, Matthew D. / Isolation and characterization of anti-monosialoganglioside monoclonal antibody 19-9 class-switch variants. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 24. pp. 8653-8657.
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