Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5

C. Morgan, J. W. Pollard, E. R. Stanley

Research output: Contribution to journalArticle

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Abstract

The SV40 transformed murine macrophage cell line, BAC1, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 132:1158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BAC1 were established. Clones ranged from being completely autonomous to being completely dependent on CSF-1 for growth. Cells of one clone (2F5), which proliferated in response to either CSF-1 or granulocyte-macrophage CSF (GM-CSF) were characterized in detail. The kinetics of receptor-mediated internalization and intracellular destruction of CSF-1 were comparable to the kinetics observed with peritoneal exudate macrophages. CSF-1 was shown to regulate cell spreading, cell survival, protein degradation, and the duration of the G1 and S phases of the cell cycle. The 2F5 clone therefore exhibits a number of CSF-1 stimulated responses and is being used for genetic and biochemical studies of CSF-1 action.

Original languageEnglish (US)
Pages (from-to)420-427
Number of pages8
JournalJournal of Cellular Physiology
Volume130
Issue number3
StatePublished - 1987

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Macrophage Colony-Stimulating Factor
Macrophages
Intercellular Signaling Peptides and Proteins
Cells
Cell Line
Clone Cells
Molecular Biology
Colony-Stimulating Factors
Signal transduction
Kinetics
G1 Phase
Peritoneal Macrophages
Exudates and Transudates
S Phase
Granulocytes
Proteolysis
Signal Transduction
Cell Survival
Cell Cycle
Degradation

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5. / Morgan, C.; Pollard, J. W.; Stanley, E. R.

In: Journal of Cellular Physiology, Vol. 130, No. 3, 1987, p. 420-427.

Research output: Contribution to journalArticle

Morgan, C. ; Pollard, J. W. ; Stanley, E. R. / Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5. In: Journal of Cellular Physiology. 1987 ; Vol. 130, No. 3. pp. 420-427.
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