Isolation and analysis of Mycobacterium tuberculosis mycolic acids.

Catherine Vilchèze; William R. Jacobs

doi:10.1002/9780471729259.mc10a03s05

Isolation and analysis of Mycobacterium tuberculosis mycolic acids.

Catherine Vilchèze, William R. Jacobs

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Mycolic acids are an important constituent of the mycobacterial cell wall. Changes in the structure or composition of mycolic acids have been associated with modification of cell wall permeability and attenuation of pathogenic Mycobacterium tuberculosis strains. This unit describes the isolation of M. tuberculosis mycolic acids and their analysis by either thin-layer chromatography or high-performance liquid chromatography. Both techniques have been extensively used to study (1) mycolic acid biosynthesis, (2) the role of mycolic acids in mycobacterial virulence, (3) the effect of antituberculosis drugs targeting the cell wall, and (4) taxonomic purposes.

Original language	English (US)
Pages (from-to)	Unit 10A.3
Journal	Current protocols in microbiology
Volume	Chapter 10
DOIs	https://doi.org/10.1002/9780471729259.mc10a03s05
State	Published - Jun 2007

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/9780471729259.mc10a03s05

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AB - Mycolic acids are an important constituent of the mycobacterial cell wall. Changes in the structure or composition of mycolic acids have been associated with modification of cell wall permeability and attenuation of pathogenic Mycobacterium tuberculosis strains. This unit describes the isolation of M. tuberculosis mycolic acids and their analysis by either thin-layer chromatography or high-performance liquid chromatography. Both techniques have been extensively used to study (1) mycolic acid biosynthesis, (2) the role of mycolic acids in mycobacterial virulence, (3) the effect of antituberculosis drugs targeting the cell wall, and (4) taxonomic purposes.

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Isolation and analysis of Mycobacterium tuberculosis mycolic acids.

Abstract

ASJC Scopus subject areas

UN SDGs

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