Isoelectric focusing without ampholyte

Simeon Pollack

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We have studied the electrophoretic mobility of proteins in polyacrylamide gels when ampholyte and buffer were excluded from the system. We found, using a weak acid and base as anolyte and catholyte respectively, that a pH gradient was generated in the gel, and that proteins focused as sharp bands.

Original languageEnglish (US)
Pages (from-to)1252-1255
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume87
Issue number4
DOIs
StatePublished - Apr 27 1979

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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