Isoelectric focusing without ampholyte

Simeon Pollack

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have studied the electrophoretic mobility of proteins in polyacrylamide gels when ampholyte and buffer were excluded from the system. We found, using a weak acid and base as anolyte and catholyte respectively, that a pH gradient was generated in the gel, and that proteins focused as sharp bands.

Original languageEnglish (US)
Pages (from-to)1252-1255
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume87
Issue number4
DOIs
StatePublished - Apr 27 1979

Fingerprint

Isoelectric Focusing
Buffers
Electrophoretic mobility
Proton-Motive Force
Proteins
Gels
Acids
polyacrylamide gels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isoelectric focusing without ampholyte. / Pollack, Simeon.

In: Biochemical and Biophysical Research Communications, Vol. 87, No. 4, 27.04.1979, p. 1252-1255.

Research output: Contribution to journalArticle

Pollack, Simeon. / Isoelectric focusing without ampholyte. In: Biochemical and Biophysical Research Communications. 1979 ; Vol. 87, No. 4. pp. 1252-1255.
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