Abstract
We have studied the electrophoretic mobility of proteins in polyacrylamide gels when ampholyte and buffer were excluded from the system. We found, using a weak acid and base as anolyte and catholyte respectively, that a pH gradient was generated in the gel, and that proteins focused as sharp bands.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1252-1255 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 87 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 27 1979 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology