IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3′-kinase

Martin G. Myers, Xiao Jian Sun, Bentley Cheatham, Bozena R. Jachna, Erin M. Glasheen, Jonathan M. Backer, Morris F. White

Research output: Contribution to journalArticle

325 Citations (Scopus)

Abstract

IRS-1 is a unique cytosolic protein that becomes tyrosine phosphorylated during insulin stimulation of intact cells and immediately associates with the phosphatidylinositol 3′-kinase (PtdIns 3′-kinase). The insulin-like growth factor-I (IGF-I) receptor also mediated the tyrosine phosphorylation of IRS-1 and increased the amount of PtdIns 3′-kinase activity bound to IRS-1 in Chinese hamster ovary cells. Purified insulin receptor and IGF-I receptor phosphorylated recombinant baculovirus-produced IRS-1 on similar sites in vitro, and phosphorylated baculovirus-produced IRS-1 bound PtdIns 3′-kinase activity from lysates of quiescent cells. Treatment of cells with IGF-I activated the PtdIns 3′-kinase, suggesting that IGF-I activates the PtdIns 3′-kinase through IRS-1 binding to p85 in a manner similar to insulin. Chinese hamster ovary cells overexpressing IRS-1 demonstrated increased tyrosine phosphorylation of IRS-1, and more PtdIns 3′-kinase activity associated with IRS-1 in these cells. These data demonstrate that IRS-1 is a common element for signal transmission by the IGF-I and insulin receptors.

Original languageEnglish (US)
Pages (from-to)1421-1430
Number of pages10
JournalEndocrinology
Volume132
Issue number4
StatePublished - Apr 1993
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Insulin-Like Growth Factor I
Insulin
IGF Type 1 Receptor
Tyrosine
Baculoviridae
Insulin Receptor
Cricetulus
Ovary
Phosphorylation

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Myers, M. G., Sun, X. J., Cheatham, B., Jachna, B. R., Glasheen, E. M., Backer, J. M., & White, M. F. (1993). IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3′-kinase. Endocrinology, 132(4), 1421-1430.

IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3′-kinase. / Myers, Martin G.; Sun, Xiao Jian; Cheatham, Bentley; Jachna, Bozena R.; Glasheen, Erin M.; Backer, Jonathan M.; White, Morris F.

In: Endocrinology, Vol. 132, No. 4, 04.1993, p. 1421-1430.

Research output: Contribution to journalArticle

Myers, MG, Sun, XJ, Cheatham, B, Jachna, BR, Glasheen, EM, Backer, JM & White, MF 1993, 'IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3′-kinase', Endocrinology, vol. 132, no. 4, pp. 1421-1430.
Myers, Martin G. ; Sun, Xiao Jian ; Cheatham, Bentley ; Jachna, Bozena R. ; Glasheen, Erin M. ; Backer, Jonathan M. ; White, Morris F. / IRS-1 is a common element in insulin and insulin-like growth factor-I signaling to the phosphatidylinositol 3′-kinase. In: Endocrinology. 1993 ; Vol. 132, No. 4. pp. 1421-1430.
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