TY - JOUR
T1 - Involvement of the calcium-independent receptor for alpha-latrotoxin in brain ischemia
AU - Bin Sun, Hui
AU - Ruan, Yiwen
AU - Xu, Zao C.
AU - Yokota, Hiroki
N1 - Funding Information:
This research was supported, in part, by grants AHA0070048 and NIH NS38053 (Z.C.X.), and Ikeda Scientific Funds (H.Y.).
PY - 2002
Y1 - 2002
N2 - Cerebral ischemia is caused by a reduced blood supply to neurons, and vulnerability to neurodegeneration varies considerably among neuronal types. In hippocampus, neurons in the CA1 region are more susceptible to ischemia-induced neuronal death than neurons in the CA3 region, and in response to transient forebrain ischemia a family of calcium-dependent receptors for alpha-latrotoxin is differentially expressed in the two regions. Here, we report that an ischemic insult up-regulated a family of calcium-independent receptors for alpha-latrotoxin (CIRL) mRNAs in CA1 neurons and down-regulated their mRNAs in CA3 neurons. Furthermore, antisense oligonucleotides complementary to CIRL-1 mRNA or CIRL-3 mRNA suppressed neuronal death associated with hypoxia in hippocampal and cortical cell cultures. The observed region-specific CIRL mRNA expression in hippocampus and an in vitro rescue experiment by antisense oligonucleotides against CIRL mRNAs suggest a functional importance of CIRL in neurodegeneration.
AB - Cerebral ischemia is caused by a reduced blood supply to neurons, and vulnerability to neurodegeneration varies considerably among neuronal types. In hippocampus, neurons in the CA1 region are more susceptible to ischemia-induced neuronal death than neurons in the CA3 region, and in response to transient forebrain ischemia a family of calcium-dependent receptors for alpha-latrotoxin is differentially expressed in the two regions. Here, we report that an ischemic insult up-regulated a family of calcium-independent receptors for alpha-latrotoxin (CIRL) mRNAs in CA1 neurons and down-regulated their mRNAs in CA3 neurons. Furthermore, antisense oligonucleotides complementary to CIRL-1 mRNA or CIRL-3 mRNA suppressed neuronal death associated with hypoxia in hippocampal and cortical cell cultures. The observed region-specific CIRL mRNA expression in hippocampus and an in vitro rescue experiment by antisense oligonucleotides against CIRL mRNAs suggest a functional importance of CIRL in neurodegeneration.
UR - http://www.scopus.com/inward/record.url?scp=0037103861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037103861&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(02)00386-8
DO - 10.1016/S0169-328X(02)00386-8
M3 - Article
C2 - 12225880
AN - SCOPUS:0037103861
SN - 0169-328X
VL - 104
SP - 246
EP - 249
JO - Brain research. Molecular brain research
JF - Brain research. Molecular brain research
IS - 2
ER -