Involvement of cytoskeleton in Junín virus entry

M. Guadalupe Martinez, Sandra M. Cordo, Nélida A. Candurra

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting main cytoskeletal components on JUNV entry into Vero cells were analyzed. Drugs that disrupted microfilaments or stabilized microtubules inhibited early steps of virus entry. In contrast, drugs that stabilized microfilaments or depolymerized microtubules were not able to block virus entry very efficiently. Furthermore, real time PCR was performed to detect viral entry and we found more than 10-fold less RNA when microfilaments were depolymerized while a 100-fold diminution was seen when microtubules were stabilized. Taken together our results demonstrate that JUNV relies on an intact actin network during early infection in Vero cells while a dynamic microtubule network is also needed. This represents an important contribution to the characterization of arenavirus multiplication cycle.

Original languageEnglish (US)
Pages (from-to)17-25
Number of pages9
JournalVirus Research
Volume138
Issue number1-2
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

Virus Internalization
Cytoskeleton
Microtubules
Actin Cytoskeleton
Vero Cells
Virus Diseases
Arenavirus
Pharmaceutical Preparations
Viruses
Clathrin
Endocytosis
Actins
Real-Time Polymerase Chain Reaction
RNA
Infection

Keywords

  • Actin
  • Cytoskeleton
  • Junín virus
  • Tubulin

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Martinez, M. G., Cordo, S. M., & Candurra, N. A. (2008). Involvement of cytoskeleton in Junín virus entry. Virus Research, 138(1-2), 17-25. https://doi.org/10.1016/j.virusres.2008.08.004

Involvement of cytoskeleton in Junín virus entry. / Martinez, M. Guadalupe; Cordo, Sandra M.; Candurra, Nélida A.

In: Virus Research, Vol. 138, No. 1-2, 12.2008, p. 17-25.

Research output: Contribution to journalArticle

Martinez, MG, Cordo, SM & Candurra, NA 2008, 'Involvement of cytoskeleton in Junín virus entry', Virus Research, vol. 138, no. 1-2, pp. 17-25. https://doi.org/10.1016/j.virusres.2008.08.004
Martinez, M. Guadalupe ; Cordo, Sandra M. ; Candurra, Nélida A. / Involvement of cytoskeleton in Junín virus entry. In: Virus Research. 2008 ; Vol. 138, No. 1-2. pp. 17-25.
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