Involvement of cellular proteins in Junin arenavirus entry

M. Guadalupe Martinez, M. Belen Forlenza, Nélida A. Candurra

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV.

Original languageEnglish (US)
Pages (from-to)866-870
Number of pages5
JournalBiotechnology Journal
Volume4
Issue number6
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Arenavirus
Proteins
Dynamin II
Clathrin
Vero Cells
Endosomes
GTP Phosphohydrolases
Actin Cytoskeleton
Microtubules
Viruses

Keywords

  • Arenavirus
  • Endocytosis
  • Junin virus

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Martinez, M. G., Forlenza, M. B., & Candurra, N. A. (2009). Involvement of cellular proteins in Junin arenavirus entry. Biotechnology Journal, 4(6), 866-870. https://doi.org/10.1002/biot.200800357

Involvement of cellular proteins in Junin arenavirus entry. / Martinez, M. Guadalupe; Forlenza, M. Belen; Candurra, Nélida A.

In: Biotechnology Journal, Vol. 4, No. 6, 2009, p. 866-870.

Research output: Contribution to journalArticle

Martinez, MG, Forlenza, MB & Candurra, NA 2009, 'Involvement of cellular proteins in Junin arenavirus entry', Biotechnology Journal, vol. 4, no. 6, pp. 866-870. https://doi.org/10.1002/biot.200800357
Martinez, M. Guadalupe ; Forlenza, M. Belen ; Candurra, Nélida A. / Involvement of cellular proteins in Junin arenavirus entry. In: Biotechnology Journal. 2009 ; Vol. 4, No. 6. pp. 866-870.
@article{c7baa23434704e328cbfefee3734c4e9,
title = "Involvement of cellular proteins in Junin arenavirus entry",
abstract = "Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV.",
keywords = "Arenavirus, Endocytosis, Junin virus",
author = "Martinez, {M. Guadalupe} and Forlenza, {M. Belen} and Candurra, {N{\'e}lida A.}",
year = "2009",
doi = "10.1002/biot.200800357",
language = "English (US)",
volume = "4",
pages = "866--870",
journal = "Biotechnology Journal",
issn = "1860-6768",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Involvement of cellular proteins in Junin arenavirus entry

AU - Martinez, M. Guadalupe

AU - Forlenza, M. Belen

AU - Candurra, Nélida A.

PY - 2009

Y1 - 2009

N2 - Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV.

AB - Junin arenavirus (JUNV) entry is dependent on clathrin-mediated pathways and it relies on the integrity of the actin cytoskeleton as well as the dynamics of microtubules. To determine the method of entry used by this human pathogen, we have demonstrated that in Vero cells JUNV is trafficked via the cellular dynamin 2 (dyn2) endocytic pathway and it is dependent on the Eps15 GTPase. In addition, we have shown that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes in its pH-dependent entry. Altogether, this study gives further inside into the endocytic pathway utilized by the arenavirus JUNV.

KW - Arenavirus

KW - Endocytosis

KW - Junin virus

UR - http://www.scopus.com/inward/record.url?scp=68049085465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68049085465&partnerID=8YFLogxK

U2 - 10.1002/biot.200800357

DO - 10.1002/biot.200800357

M3 - Article

VL - 4

SP - 866

EP - 870

JO - Biotechnology Journal

JF - Biotechnology Journal

SN - 1860-6768

IS - 6

ER -