TY - JOUR
T1 - Investigation of human urine for genomic sequences of the primate polyomaviruses simian virus 40, BK virus, and JC virus
AU - Shah, K. V.
AU - Daniel, R. W.
AU - Strickler, H. D.
AU - Goedert, J. J.
N1 - Funding Information:
Received 28 April 1997; revised 21 July 1997. Presented in part: CBER-NCI-NICHD-NIP-NVPO Workshop on Simian Virus 40 (SV40): A Possible Human Polyomavirus, 27–28 January 1997, Bethesda, Maryland. Informed consent was obtained from patients, and human experimentation guidelines of the US Department of Health and Human Services were followed in this investigation. Grant support: NIH (contract CP-40521). Reprints or correspondence: Dr. Keerti V. Shah, Dept. of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205.
PY - 1997
Y1 - 1997
N2 - Recent reports of the detection of simian virus 40 (SV40) nucleotide sequences in ependymomas, choroid plexus tumors, osteosarcomas, and mesotheliomas have raised the possibility that SV40, which naturally infects Asian macaques, is circulating among humans. This possibility was examined by performing polymerase chain reaction assays on urine samples of 166 homosexual men, 88 of them human immunodeficiency virus (HIV)-seropositive, for genomic sequences of SV40 as well as of human polyomaviroses BK virus (BKV) and JC virus (JCV). Tests with masked urine specimens spiked with SV40- transformed cells were included to monitor the SV40 assay, SV40, BKV, and JCV sequences were identified, respectively, in 0, 14%, and 34% of the urine specimens. JCV viruria was far more common (37%) than BKV viruria (5%) in HIV-seronegative persons. HIV infection and more severe immunosuppression were associated with a higher frequency of BKV viruria. In summary, SV40 viruria was not detected among homosexual men who shed human polyomaviruses at a high frequency.
AB - Recent reports of the detection of simian virus 40 (SV40) nucleotide sequences in ependymomas, choroid plexus tumors, osteosarcomas, and mesotheliomas have raised the possibility that SV40, which naturally infects Asian macaques, is circulating among humans. This possibility was examined by performing polymerase chain reaction assays on urine samples of 166 homosexual men, 88 of them human immunodeficiency virus (HIV)-seropositive, for genomic sequences of SV40 as well as of human polyomaviroses BK virus (BKV) and JC virus (JCV). Tests with masked urine specimens spiked with SV40- transformed cells were included to monitor the SV40 assay, SV40, BKV, and JCV sequences were identified, respectively, in 0, 14%, and 34% of the urine specimens. JCV viruria was far more common (37%) than BKV viruria (5%) in HIV-seronegative persons. HIV infection and more severe immunosuppression were associated with a higher frequency of BKV viruria. In summary, SV40 viruria was not detected among homosexual men who shed human polyomaviruses at a high frequency.
UR - http://www.scopus.com/inward/record.url?scp=0030722275&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030722275&partnerID=8YFLogxK
U2 - 10.1086/517340
DO - 10.1086/517340
M3 - Article
C2 - 9395377
AN - SCOPUS:0030722275
SN - 0022-1899
VL - 176
SP - 1618
EP - 1621
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -
