Investigation of human urine for genomic sequences of the primate polyomaviruses simian virus 40, BK virus, and JC virus

K. V. Shah, R. W. Daniel, Howard Strickler, J. J. Goedert

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Recent reports of the detection of simian virus 40 (SV40) nucleotide sequences in ependymomas, choroid plexus tumors, osteosarcomas, and mesotheliomas have raised the possibility that SV40, which naturally infects Asian macaques, is circulating among humans. This possibility was examined by performing polymerase chain reaction assays on urine samples of 166 homosexual men, 88 of them human immunodeficiency virus (HIV)-seropositive, for genomic sequences of SV40 as well as of human polyomaviroses BK virus (BKV) and JC virus (JCV). Tests with masked urine specimens spiked with SV40- transformed cells were included to monitor the SV40 assay, SV40, BKV, and JCV sequences were identified, respectively, in 0, 14%, and 34% of the urine specimens. JCV viruria was far more common (37%) than BKV viruria (5%) in HIV-seronegative persons. HIV infection and more severe immunosuppression were associated with a higher frequency of BKV viruria. In summary, SV40 viruria was not detected among homosexual men who shed human polyomaviruses at a high frequency.

Original languageEnglish (US)
Pages (from-to)1618-1621
Number of pages4
JournalJournal of Infectious Diseases
Volume176
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

BK Virus
JC Virus
Polyomavirus
Simian virus 40
Primates
Urine
HIV
Choroid Plexus Neoplasms
Ependymoma
Mesothelioma
Macaca
Virus Diseases
Osteosarcoma
Immunosuppression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Investigation of human urine for genomic sequences of the primate polyomaviruses simian virus 40, BK virus, and JC virus. / Shah, K. V.; Daniel, R. W.; Strickler, Howard; Goedert, J. J.

In: Journal of Infectious Diseases, Vol. 176, No. 6, 1997, p. 1618-1621.

Research output: Contribution to journalArticle

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