Inverted repeats are necessary for circularization of the mouse testis Sry transcript

Robert A. Dubin, Manija A. Kazmi, Harry Ostrer

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Circular non-polyadenylated RNA molecules have been identified as stable transcription products of the human ETS-1 and mouse Sry genes. RNA circularization has been proposed to require two steps. The first step utilizes intramolecular base pairing to produce a transient stem-loop structure. The second step involves splicing a downstream donor splice site (DSS) to a now closely appositioned upstream acceptor splice site (ASS) within the loop. We demonstrate that the presence of long inverted repeats (IR) flanking the mouse Sry gene leads to the formation of the Sry circular transcript in cultured cells. Circularization requires the presence of both IR. As few as 400 complementary nt are necessary for this process. The presence of the IR does not significantly stimulate intermolecular annealing and trans-splicing in vivo.

Original languageEnglish (US)
Pages (from-to)245-248
Number of pages4
JournalGene
Volume167
Issue number1-2
DOIs
StatePublished - Dec 29 1995
Externally publishedYes

Keywords

  • RNA processing
  • Recombinant DNA
  • reverse transcription PCR
  • sex determination
  • splicing

ASJC Scopus subject areas

  • Genetics

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