Intrinsic fluorescence emission of intact oxy hemoglobins

Rhoda Elison Hirsch, R. Suzanne Zukin, Ronald L. Nagel

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

Fluorescence has not been previously detected in intact hemoproteins. We have been able to measure significant fluorescence emission in purified oxy HbA using front-face fluorometry. The excitation maximum (293 nm), the emission maximum (325 nm) and the fluorescence spectra of Hb Rothschild (β 37 Trp → Arg) allows us to conclude that β 37 Trp is primarily responsible for the fluorescence signal of HbA. We propose that this intrinsic fluorescence of hemoglobin may be used as a probe to study conformational changes in hemoglobin and possibly other heme-containing proteins.

Original languageEnglish (US)
Pages (from-to)432-439
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume93
Issue number2
DOIs
StatePublished - Mar 28 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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