Intravital imaging of metastatic behavior through a mammary imaging window

Dmitriy Kedrin, Bojana Gligorijevic, Jeffrey Wyckoff, Vladislav Verkhusha, John S. Condeelis, Jeffrey E. Segall, Jacco van Rheenen

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

We report a technique to evaluate the same tumor microenvironment over multiple intravital imaging sessions in living mice. We optically marked individual tumor cells expressing photoswitchable proteins in an orthotopic mammary carcinoma and followed them for extended periods through a mammary imaging window. We found that two distinct microenvironments in the same orthotopic mammary tumor affected differently the invasion and intravasation of tumor cells.

Original languageEnglish (US)
Pages (from-to)1019-1021
Number of pages3
JournalNature Methods
Volume5
Issue number12
DOIs
StatePublished - 2008

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Tumors
Breast
Breast Neoplasms
Imaging techniques
Tumor Microenvironment
Neoplasms
Cells
Proteins
Intravital Microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Intravital imaging of metastatic behavior through a mammary imaging window. / Kedrin, Dmitriy; Gligorijevic, Bojana; Wyckoff, Jeffrey; Verkhusha, Vladislav; Condeelis, John S.; Segall, Jeffrey E.; van Rheenen, Jacco.

In: Nature Methods, Vol. 5, No. 12, 2008, p. 1019-1021.

Research output: Contribution to journalArticle

Kedrin, Dmitriy ; Gligorijevic, Bojana ; Wyckoff, Jeffrey ; Verkhusha, Vladislav ; Condeelis, John S. ; Segall, Jeffrey E. ; van Rheenen, Jacco. / Intravital imaging of metastatic behavior through a mammary imaging window. In: Nature Methods. 2008 ; Vol. 5, No. 12. pp. 1019-1021.
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