Intraventricular hemorrhage induces deposition of proteoglycans in premature rabbits, but their in vivo degradation with chondroitinase does not restore myelination, ventricle size and neurological recovery

Govindaiah Vinukonda, Muhammad T. Zia, Bala B R Bhimavarapu, Furong Hu, Michelle Feinberg, Aqiba Bokhari, Zoltan Ungvari, Victor A. Fried, Praveen Ballabh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Intraventricular hemorrhage (IVH) results in white matter injury and hydrocephalus in premature infants. Chondroitin sulfate proteoglycans (CSPGs)-neuorcan, brevican, versican, aggrecan and phosphacan-are unregulated in the extracellular matrix after brain injury, and their degradation enhances plasticity of the brain. Therefore, we hypothesized that CSPG levels were elevated in the forebrain of premature infants with IVH and that in vivo degradation of CSPGs would enhance maturation of oligodendrocyte, augment myelination, promote neurological recovery, and minimize hydrocephalus. We found that levels of neurocan, brevican, aggrecan, phosphacan, and versican were elevated, whereas NG2 expression was reduced in premature rabbit pups and human infants with IVH compared to controls. Intracerebroventricular chondroitinase ABC (ChABC) reduced the expression of neuorcan, brevican, versican and aggrecan, but not NG2. However, ChABC treatment did not enhance maturation of oligodendrocytes, myelination, or neurological recovery in the pups with IVH. Moreover, ChABC did not reduce gliosis or ventriculomegaly. Our results demonstrate that IVH induces distinct changes in the components of CSPGs, and that reversing these changes by in vivo ChABC treatment neither promotes clinical recovery, myelination, nor reduces ventriculomegaly in preterm rabbit pups.

Original languageEnglish (US)
Pages (from-to)630-644
Number of pages15
JournalExperimental Neurology
Volume247
DOIs
StatePublished - Sep 2013
Externally publishedYes

Fingerprint

Chondroitinases and Chondroitin Lyases
Chondroitin ABC Lyase
Chondroitin Sulfate Proteoglycans
Proteoglycans
Brevican
Versicans
Aggrecans
Hemorrhage
Rabbits
Class 5 Receptor-Like Protein Tyrosine Phosphatases
Oligodendroglia
Hydrocephalus
Premature Infants
Neurocan
Gliosis
Prosencephalon
Brain Injuries
Extracellular Matrix
Wounds and Injuries
Brain

Keywords

  • Aggrecan
  • Brevican
  • Chondroitin sulfate proteoglycans
  • Chondroitinase ABC
  • Myelination
  • Neuorcan
  • Oligodendrocyte
  • Phosphacan
  • Versican

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Intraventricular hemorrhage induces deposition of proteoglycans in premature rabbits, but their in vivo degradation with chondroitinase does not restore myelination, ventricle size and neurological recovery. / Vinukonda, Govindaiah; Zia, Muhammad T.; Bhimavarapu, Bala B R; Hu, Furong; Feinberg, Michelle; Bokhari, Aqiba; Ungvari, Zoltan; Fried, Victor A.; Ballabh, Praveen.

In: Experimental Neurology, Vol. 247, 09.2013, p. 630-644.

Research output: Contribution to journalArticle

Vinukonda, Govindaiah ; Zia, Muhammad T. ; Bhimavarapu, Bala B R ; Hu, Furong ; Feinberg, Michelle ; Bokhari, Aqiba ; Ungvari, Zoltan ; Fried, Victor A. ; Ballabh, Praveen. / Intraventricular hemorrhage induces deposition of proteoglycans in premature rabbits, but their in vivo degradation with chondroitinase does not restore myelination, ventricle size and neurological recovery. In: Experimental Neurology. 2013 ; Vol. 247. pp. 630-644.
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AU - Zia, Muhammad T.

AU - Bhimavarapu, Bala B R

AU - Hu, Furong

AU - Feinberg, Michelle

AU - Bokhari, Aqiba

AU - Ungvari, Zoltan

AU - Fried, Victor A.

AU - Ballabh, Praveen

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