Intravenous immunoglobulins reverse acute vaso-occlusive crises in sickle cell mice through rapid inhibition of neutrophil adhesion

Jungshan Chang, Patricia A. Shi, Elaine Y. Chiang, Paul S. Frenette

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Previous studies using intravital microscopy in a sickle cell disease (SCD) mouse model suggest that adherent white blood cells (WBCs) play a key role in vasoocclusion by capturing circulating red blood cells (RBCs) in venules. Commercial intravenous immunoglobulin (IVIG) given before the inflammatory stimuli increased microcirculatory blood flow and survival. To mimic the clinical situation in which SCD patients seek medical attention after the onset of symptoms, we developed an in vivo model in which the therapeutic intervention (eg, IVIG) was administered after in the inflammatory challenge. In this setting, IVIG rapidly (< 10 minutes) reduced adherent leukocyte numbers and dramatically inhibited interactions between RBCs and WBCs, resulting in improved microcirculatory blood flow and survival of sickle cell "Berkeley" mice. Longer survival correlated positively with blood flow (P = .001) and negatively with the number of adherent leukocytes (P = .001) and RBC-WBC interactions (P = .002). Using multichannel digital fluorescence videomicroscopy, we found that IVIG affected specifically the recruitment of neutrophils. Moreover, further analyses of leukocyte behavior revealed that IVIG significantly increased rolling velocities, indicating that it alters adhesion pathways involved in slow rolling. These data suggest that the potential therapeutic benefits of IVIG in SCD crises should be evaluated in a clinical trial.

Original languageEnglish (US)
Pages (from-to)915-923
Number of pages9
JournalBlood
Volume111
Issue number2
DOIs
StatePublished - Jan 15 2008
Externally publishedYes

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Intravenous Immunoglobulins
Neutrophils
Blood
Adhesion
Sickle Cell Anemia
Leukocytes
Erythrocytes
Leukocyte Count
Video Microscopy
Survival
Venules
Neutrophil Infiltration
Cells
Cell Communication
Inhibition (Psychology)
Cell Survival
Fluorescence
Clinical Trials
Therapeutics

ASJC Scopus subject areas

  • Hematology

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Intravenous immunoglobulins reverse acute vaso-occlusive crises in sickle cell mice through rapid inhibition of neutrophil adhesion. / Chang, Jungshan; Shi, Patricia A.; Chiang, Elaine Y.; Frenette, Paul S.

In: Blood, Vol. 111, No. 2, 15.01.2008, p. 915-923.

Research output: Contribution to journalArticle

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