Intravascular Ultrasound Characterization of a Tissue-Engineered Vascular Graft in an Ovine Model

Victoria K. Pepper; Elizabeth S. Clark; Cameron A. Best; Ekene A. Onwuka; Tadahisa Sugiura; Eric D. Heuer; Lilamarie E. Moko; Shinka Miyamoto; Hideki Miyachi; Darren P. Berman; Sharon L. Cheatham; Joanne L. Chisolm; Toshiharu Shinoka; Christopher K. Breuer; John P. Cheatham

doi:10.1007/s12265-016-9725-x

Intravascular Ultrasound Characterization of a Tissue-Engineered Vascular Graft in an Ovine Model

Victoria K. Pepper, Elizabeth S. Clark, Cameron A. Best, Ekene A. Onwuka, Tadahisa Sugiura, Eric D. Heuer, Lilamarie E. Moko, Shinka Miyamoto, Hideki Miyachi, Darren P. Berman, Sharon L. Cheatham, Joanne L. Chisolm, Toshiharu Shinoka, Christopher K. Breuer, John P. Cheatham

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model. Eight sheep underwent implantation of TEVGs either unseeded or seeded with bone marrow-derived mononuclear cells. Angiography, IVUS, and histology were directly compared. Endothelium, tunica media, and graft were identifiable on IVUS and histology at multiple time points. There was strong agreement between IVUS and angiography for evaluation of luminal diameter. IVUS offers a valuable tool to evaluate the changes within TEVGs, and clinical translation of this application is warranted.

Original language	English (US)
Pages (from-to)	128-138
Number of pages	11
Journal	Journal of Cardiovascular Translational Research
Volume	10
Issue number	2
DOIs	https://doi.org/10.1007/s12265-016-9725-x
State	Published - Apr 1 2017
Externally published	Yes

Keywords

Angiography
IVUS
Intravascular ultrasound
Neotissue
Stenosis
TEVG
Tissue-engineered vascular graft

ASJC Scopus subject areas

Molecular Medicine
Genetics
Pharmaceutical Science
Cardiology and Cardiovascular Medicine
Genetics(clinical)

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10.1007/s12265-016-9725-x

Cite this

Pepper, V. K., Clark, E. S., Best, C. A., Onwuka, E. A., Sugiura, T., Heuer, E. D., Moko, L. E., Miyamoto, S., Miyachi, H., Berman, D. P., Cheatham, S. L., Chisolm, J. L., Shinoka, T., Breuer, C. K., & Cheatham, J. P. (2017). Intravascular Ultrasound Characterization of a Tissue-Engineered Vascular Graft in an Ovine Model. Journal of Cardiovascular Translational Research, 10(2), 128-138. https://doi.org/10.1007/s12265-016-9725-x

Pepper, VK, Clark, ES, Best, CA, Onwuka, EA, Sugiura, T, Heuer, ED, Moko, LE, Miyamoto, S, Miyachi, H, Berman, DP, Cheatham, SL, Chisolm, JL, Shinoka, T, Breuer, CK & Cheatham, JP 2017, 'Intravascular Ultrasound Characterization of a Tissue-Engineered Vascular Graft in an Ovine Model', Journal of Cardiovascular Translational Research, vol. 10, no. 2, pp. 128-138. https://doi.org/10.1007/s12265-016-9725-x

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abstract = "Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model. Eight sheep underwent implantation of TEVGs either unseeded or seeded with bone marrow-derived mononuclear cells. Angiography, IVUS, and histology were directly compared. Endothelium, tunica media, and graft were identifiable on IVUS and histology at multiple time points. There was strong agreement between IVUS and angiography for evaluation of luminal diameter. IVUS offers a valuable tool to evaluate the changes within TEVGs, and clinical translation of this application is warranted.",

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AU - Sugiura, Tadahisa

AU - Heuer, Eric D.

AU - Moko, Lilamarie E.

AU - Miyamoto, Shinka

AU - Miyachi, Hideki

AU - Berman, Darren P.

AU - Cheatham, Sharon L.

AU - Chisolm, Joanne L.

AU - Shinoka, Toshiharu

AU - Breuer, Christopher K.

AU - Cheatham, John P.

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AB - Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model. Eight sheep underwent implantation of TEVGs either unseeded or seeded with bone marrow-derived mononuclear cells. Angiography, IVUS, and histology were directly compared. Endothelium, tunica media, and graft were identifiable on IVUS and histology at multiple time points. There was strong agreement between IVUS and angiography for evaluation of luminal diameter. IVUS offers a valuable tool to evaluate the changes within TEVGs, and clinical translation of this application is warranted.

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Intravascular Ultrasound Characterization of a Tissue-Engineered Vascular Graft in an Ovine Model

Abstract

Keywords

ASJC Scopus subject areas

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