TY - JOUR
T1 - Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection
AU - De Matos Guedes, Herbert Leonel
AU - Da Silva Costa, Beatriz Lilian
AU - Chaves, Suzana Passos
AU - De Oliveira Gomes, Daniel Cláudio
AU - Nosanchuk, Joshua Daniel
AU - De Simone, Salvatore Giovanni
AU - Rossi-Bergmann, Bartira
N1 - Publisher Copyright:
© 2014 de Matos Guedes et al.; licensee BioMed Central Ltd.
PY - 2014/9/19
Y1 - 2014/9/19
N2 - Conclusion: This study indicates that extracellular serine proteases of L. amazonensis are strong candidates for a more defined intranasal vaccine against cutaneous leishmaniasis.Background: Previously, we demonstrated that unlike subcutaneous or intramuscular vaccination, intranasal vaccination of BALB/c mice with whole Leishmania amazonensis antigens leads to protection against cutaneous leishmaniasis. Here, the role of parasite serine proteases in the protective immunity was investigated.Findings. Serine Proteases were partially purified from both soluble (LaSP-Sol) and extracellular (LaSP-Ex) Leishmania amazonensis promastigote extracts by aprotinin-agarose chromatography. BALB/c mice were intranasally immunized with LaSP-Sol and LaSP-Ex prior to infection with L. amazonensis. LaSP-Ex but not LaSP-Sol vaccination led to significantly smaller lesions and parasite burdens as compared with non-vaccinated controls. Protection was accompanied by systemic Th1 polarization with increased IFN-γ and decreased IL-4 and IL-10 splenic production. Likewise, increased production of IFN-γ, IL-12 and IL-4 concomitant with decreased TGF-β and TNF-α was locally observed in the infected footpad.
AB - Conclusion: This study indicates that extracellular serine proteases of L. amazonensis are strong candidates for a more defined intranasal vaccine against cutaneous leishmaniasis.Background: Previously, we demonstrated that unlike subcutaneous or intramuscular vaccination, intranasal vaccination of BALB/c mice with whole Leishmania amazonensis antigens leads to protection against cutaneous leishmaniasis. Here, the role of parasite serine proteases in the protective immunity was investigated.Findings. Serine Proteases were partially purified from both soluble (LaSP-Sol) and extracellular (LaSP-Ex) Leishmania amazonensis promastigote extracts by aprotinin-agarose chromatography. BALB/c mice were intranasally immunized with LaSP-Sol and LaSP-Ex prior to infection with L. amazonensis. LaSP-Ex but not LaSP-Sol vaccination led to significantly smaller lesions and parasite burdens as compared with non-vaccinated controls. Protection was accompanied by systemic Th1 polarization with increased IFN-γ and decreased IL-4 and IL-10 splenic production. Likewise, increased production of IFN-γ, IL-12 and IL-4 concomitant with decreased TGF-β and TNF-α was locally observed in the infected footpad.
KW - Extracellular serine proteases
KW - Intranasal route
KW - Leishmania amazonensis
KW - Mucosal vaccine
KW - Murine cutaneous leishmaniasis
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U2 - 10.1186/1756-3305-7-448
DO - 10.1186/1756-3305-7-448
M3 - Article
C2 - 25239157
AN - SCOPUS:84907912029
SN - 1756-3305
VL - 7
JO - Parasites and Vectors
JF - Parasites and Vectors
IS - 1
M1 - 448
ER -
