Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection

Herbert Leonel De Matos Guedes, Beatriz Lilian Da Silva Costa, Suzana Passos Chaves, Daniel Cláudio De Oliveira Gomes, Joshua Daniel Nosanchuk, Salvatore Giovanni De Simone, Bartira Rossi-Bergmann

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Conclusion: This study indicates that extracellular serine proteases of L. amazonensis are strong candidates for a more defined intranasal vaccine against cutaneous leishmaniasis.

Background: Previously, we demonstrated that unlike subcutaneous or intramuscular vaccination, intranasal vaccination of BALB/c mice with whole Leishmania amazonensis antigens leads to protection against cutaneous leishmaniasis. Here, the role of parasite serine proteases in the protective immunity was investigated.

Findings. Serine Proteases were partially purified from both soluble (LaSP-Sol) and extracellular (LaSP-Ex) Leishmania amazonensis promastigote extracts by aprotinin-agarose chromatography. BALB/c mice were intranasally immunized with LaSP-Sol and LaSP-Ex prior to infection with L. amazonensis. LaSP-Ex but not LaSP-Sol vaccination led to significantly smaller lesions and parasite burdens as compared with non-vaccinated controls. Protection was accompanied by systemic Th1 polarization with increased IFN-γ and decreased IL-4 and IL-10 splenic production. Likewise, increased production of IFN-γ, IL-12 and IL-4 concomitant with decreased TGF-β and TNF-α was locally observed in the infected footpad.

Original languageEnglish (US)
Article number448
JournalParasites and Vectors
Volume7
Issue number1
DOIs
StatePublished - Sep 19 2014

Keywords

  • Extracellular serine proteases
  • Intranasal route
  • Leishmania amazonensis
  • Mucosal vaccine
  • Murine cutaneous leishmaniasis

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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  • Cite this

    De Matos Guedes, H. L., Da Silva Costa, B. L., Chaves, S. P., De Oliveira Gomes, D. C., Nosanchuk, J. D., De Simone, S. G., & Rossi-Bergmann, B. (2014). Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection. Parasites and Vectors, 7(1), [448]. https://doi.org/10.1186/1756-3305-7-448