Intramolecular Strain Coordinates Kinesin Stepping Behavior along Microtubules

Ahmet Yildiz, Michio Tomishige, Arne Gennerich, Ronald D. Vale

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

Kinesin advances 8 nm along a microtubule per ATP hydrolyzed, but the mechanism responsible for coordinating the enzymatic cycles of kinesin's two identical motor domains remains unresolved. Here, we have tested whether such coordination is mediated by intramolecular tension generated by the "neck linkers," mechanical elements that span between the motor domains. When tension is reduced by extending the neck linkers with artificial peptides, the coupling between ATP hydrolysis and forward stepping is impaired and motor's velocity decreases as a consequence. However, speed recovers to nearly normal levels when external tension is applied by an optical trap. Remarkably, external load also induces bidirectional stepping of an immotile kinesin that lacks its mechanical element (neck linker) and fuel (ATP). Our results indicate that the kinesin motor domain senses and responds to strain in a manner that facilitates its plus-end-directed stepping and communication between its two motor domains.

Original languageEnglish (US)
Pages (from-to)1030-1041
Number of pages12
JournalCell
Volume134
Issue number6
DOIs
StatePublished - Sep 19 2008
Externally publishedYes

Fingerprint

Kinesin
Microtubules
Neck
Adenosine Triphosphate
Optical Tweezers
Hydrolysis
Peptides
Communication

Keywords

  • CELLBIO
  • CHEMBIO
  • PROTEINS

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Intramolecular Strain Coordinates Kinesin Stepping Behavior along Microtubules. / Yildiz, Ahmet; Tomishige, Michio; Gennerich, Arne; Vale, Ronald D.

In: Cell, Vol. 134, No. 6, 19.09.2008, p. 1030-1041.

Research output: Contribution to journalArticle

Yildiz, Ahmet ; Tomishige, Michio ; Gennerich, Arne ; Vale, Ronald D. / Intramolecular Strain Coordinates Kinesin Stepping Behavior along Microtubules. In: Cell. 2008 ; Vol. 134, No. 6. pp. 1030-1041.
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