Intramolecular crosslinking of hemoglobin A by sulfosuccinimidyl suberate: Application of crosslinked protein as a blood substitute

B. N. Manjula, A. S. Acharya

Research output: Contribution to journalConference article


Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed' ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the appropriate length of the alkyl chain optimal for the crosslinking reaction.

Original languageEnglish (US)
Pages (from-to)A129
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Issue number5
Publication statusPublished - Nov 1 1994
EventProceedings of the 11th Congress of the International Society for Artificial Cells, Blood Substitutes and Immobilization Biotechnology, (ISABI) - Boston, MA, USA
Duration: Jul 24 1994Jul 27 1994


ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

Cite this