Intrahepatic levels of CXCR3-associated chemokines correlate with liver inflammation and fibrosis in chronic hepatitis C

Marija Zeremski, Lydia M. Petrovic, Luis Chiriboga, Queenie B. Brown, Herman T. Yee, Milan Kinkhabwala, Ira M. Jacobson, Rositsa Dimova, Marianthi Markatou, Andrew H. Talal

Research output: Contribution to journalArticle

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Abstract

Chemokines, chemotactic cytokines, may promote hepatic inflammation in chronic hepatitis C virus (HCV) infection through the recruitment of lymphocytes to the liver parenchyma. We evaluated the association between inflammation and fibrosis and CXCR3-associated chemokines, interferon-γ (IFN-γ)-inducible protein 10 (IP-10/CXCL10), monokine induced by IFN-γ(Mig/CXCL9), and interferon-inducible T cell α chemoattractant (I-TAC/CXCL11), in HCV infection. Intrahepatic mRNA expression of these chemokines was analyzed in 106 chronic HCV-infected patients by real-time PCR. The intrahepatic localization of chemokine producer cells and CXCR3+ lymphocytes was determined in selected patients by immunohistochemistry. We found elevated intrahepatic mRNA expression of all three chemokines, most markedly CXCL10, in chronic HCV-infected patients with higher necroinflammation and fibrosis. By multivariable multivariate analysis, intrahepatic CXCL10 mRNA expression levels were significantly associated with lobular necroinflammatory grade and HCV genotype 1. In the lobular region, CXCL10-expressing and CXCL9-expressing hepatocytes predominated in areas with necroinflammation. Strong CXCL11 expression was observed in almost all portal tracts, whereas CXCL9 expression varied considerably among portal tracts in the same individual. Most intrahepatic lymphocytes express the CXCR3 receptor, and the number of CXCR3+ lymphocytes was increased in patients with advanced necroinflammation. Conclusion: These findings suggest that the CXCR3-associated chemokines, particularly CXCL10, may play an important role in the development of necroinflammation and fibrosis in the liver parenchyma in chronic HCV infection.

Original languageEnglish (US)
Pages (from-to)1440-1450
Number of pages11
JournalHepatology
Volume48
Issue number5
DOIs
StatePublished - Nov 2008

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Chronic Hepatitis C
Chemokines
Hepacivirus
Liver Cirrhosis
Inflammation
Virus Diseases
Chemokine CXCL10
Lymphocytes
Messenger RNA
Interferons
Chemokine CXCL11
Fibrosis
CXCR3 Receptors
Monokines
Liver
Lymphocyte Count
Real-Time Polymerase Chain Reaction
Hepatocytes
Multivariate Analysis
Immunohistochemistry

ASJC Scopus subject areas

  • Hepatology
  • Medicine(all)

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Intrahepatic levels of CXCR3-associated chemokines correlate with liver inflammation and fibrosis in chronic hepatitis C. / Zeremski, Marija; Petrovic, Lydia M.; Chiriboga, Luis; Brown, Queenie B.; Yee, Herman T.; Kinkhabwala, Milan; Jacobson, Ira M.; Dimova, Rositsa; Markatou, Marianthi; Talal, Andrew H.

In: Hepatology, Vol. 48, No. 5, 11.2008, p. 1440-1450.

Research output: Contribution to journalArticle

Zeremski, M, Petrovic, LM, Chiriboga, L, Brown, QB, Yee, HT, Kinkhabwala, M, Jacobson, IM, Dimova, R, Markatou, M & Talal, AH 2008, 'Intrahepatic levels of CXCR3-associated chemokines correlate with liver inflammation and fibrosis in chronic hepatitis C', Hepatology, vol. 48, no. 5, pp. 1440-1450. https://doi.org/10.1002/hep.22500
Zeremski, Marija ; Petrovic, Lydia M. ; Chiriboga, Luis ; Brown, Queenie B. ; Yee, Herman T. ; Kinkhabwala, Milan ; Jacobson, Ira M. ; Dimova, Rositsa ; Markatou, Marianthi ; Talal, Andrew H. / Intrahepatic levels of CXCR3-associated chemokines correlate with liver inflammation and fibrosis in chronic hepatitis C. In: Hepatology. 2008 ; Vol. 48, No. 5. pp. 1440-1450.
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abstract = "Chemokines, chemotactic cytokines, may promote hepatic inflammation in chronic hepatitis C virus (HCV) infection through the recruitment of lymphocytes to the liver parenchyma. We evaluated the association between inflammation and fibrosis and CXCR3-associated chemokines, interferon-γ (IFN-γ)-inducible protein 10 (IP-10/CXCL10), monokine induced by IFN-γ(Mig/CXCL9), and interferon-inducible T cell α chemoattractant (I-TAC/CXCL11), in HCV infection. Intrahepatic mRNA expression of these chemokines was analyzed in 106 chronic HCV-infected patients by real-time PCR. The intrahepatic localization of chemokine producer cells and CXCR3+ lymphocytes was determined in selected patients by immunohistochemistry. We found elevated intrahepatic mRNA expression of all three chemokines, most markedly CXCL10, in chronic HCV-infected patients with higher necroinflammation and fibrosis. By multivariable multivariate analysis, intrahepatic CXCL10 mRNA expression levels were significantly associated with lobular necroinflammatory grade and HCV genotype 1. In the lobular region, CXCL10-expressing and CXCL9-expressing hepatocytes predominated in areas with necroinflammation. Strong CXCL11 expression was observed in almost all portal tracts, whereas CXCL9 expression varied considerably among portal tracts in the same individual. Most intrahepatic lymphocytes express the CXCR3 receptor, and the number of CXCR3+ lymphocytes was increased in patients with advanced necroinflammation. Conclusion: These findings suggest that the CXCR3-associated chemokines, particularly CXCL10, may play an important role in the development of necroinflammation and fibrosis in the liver parenchyma in chronic HCV infection.",
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AU - Zeremski, Marija

AU - Petrovic, Lydia M.

AU - Chiriboga, Luis

AU - Brown, Queenie B.

AU - Yee, Herman T.

AU - Kinkhabwala, Milan

AU - Jacobson, Ira M.

AU - Dimova, Rositsa

AU - Markatou, Marianthi

AU - Talal, Andrew H.

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N2 - Chemokines, chemotactic cytokines, may promote hepatic inflammation in chronic hepatitis C virus (HCV) infection through the recruitment of lymphocytes to the liver parenchyma. We evaluated the association between inflammation and fibrosis and CXCR3-associated chemokines, interferon-γ (IFN-γ)-inducible protein 10 (IP-10/CXCL10), monokine induced by IFN-γ(Mig/CXCL9), and interferon-inducible T cell α chemoattractant (I-TAC/CXCL11), in HCV infection. Intrahepatic mRNA expression of these chemokines was analyzed in 106 chronic HCV-infected patients by real-time PCR. The intrahepatic localization of chemokine producer cells and CXCR3+ lymphocytes was determined in selected patients by immunohistochemistry. We found elevated intrahepatic mRNA expression of all three chemokines, most markedly CXCL10, in chronic HCV-infected patients with higher necroinflammation and fibrosis. By multivariable multivariate analysis, intrahepatic CXCL10 mRNA expression levels were significantly associated with lobular necroinflammatory grade and HCV genotype 1. In the lobular region, CXCL10-expressing and CXCL9-expressing hepatocytes predominated in areas with necroinflammation. Strong CXCL11 expression was observed in almost all portal tracts, whereas CXCL9 expression varied considerably among portal tracts in the same individual. Most intrahepatic lymphocytes express the CXCR3 receptor, and the number of CXCR3+ lymphocytes was increased in patients with advanced necroinflammation. Conclusion: These findings suggest that the CXCR3-associated chemokines, particularly CXCL10, may play an important role in the development of necroinflammation and fibrosis in the liver parenchyma in chronic HCV infection.

AB - Chemokines, chemotactic cytokines, may promote hepatic inflammation in chronic hepatitis C virus (HCV) infection through the recruitment of lymphocytes to the liver parenchyma. We evaluated the association between inflammation and fibrosis and CXCR3-associated chemokines, interferon-γ (IFN-γ)-inducible protein 10 (IP-10/CXCL10), monokine induced by IFN-γ(Mig/CXCL9), and interferon-inducible T cell α chemoattractant (I-TAC/CXCL11), in HCV infection. Intrahepatic mRNA expression of these chemokines was analyzed in 106 chronic HCV-infected patients by real-time PCR. The intrahepatic localization of chemokine producer cells and CXCR3+ lymphocytes was determined in selected patients by immunohistochemistry. We found elevated intrahepatic mRNA expression of all three chemokines, most markedly CXCL10, in chronic HCV-infected patients with higher necroinflammation and fibrosis. By multivariable multivariate analysis, intrahepatic CXCL10 mRNA expression levels were significantly associated with lobular necroinflammatory grade and HCV genotype 1. In the lobular region, CXCL10-expressing and CXCL9-expressing hepatocytes predominated in areas with necroinflammation. Strong CXCL11 expression was observed in almost all portal tracts, whereas CXCL9 expression varied considerably among portal tracts in the same individual. Most intrahepatic lymphocytes express the CXCR3 receptor, and the number of CXCR3+ lymphocytes was increased in patients with advanced necroinflammation. Conclusion: These findings suggest that the CXCR3-associated chemokines, particularly CXCL10, may play an important role in the development of necroinflammation and fibrosis in the liver parenchyma in chronic HCV infection.

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