Intraflagellar transport drives flagellar surface motility

Sheng Min Shih, Benjamin D. Engel, Fatih Kocabas, Thomas Bilyard, Arne Gennerich, Wallace F. Marshall, Ahmet Yildiz

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46 Scopus citations

Abstract

The assembly and maintenance of all cilia and flagella require intraflagellar transport (IFT) along the axoneme. IFT has been implicated in sensory and motile ciliary functions, but the mechanisms of this relationship remain unclear. Here, we used Chlamydomonas flagellar surface motility (FSM) as a model to test whether IFT provides force for gliding of cells across solid surfaces. We show that IFT trains are coupled to flagellar membrane glycoproteins (FMGs) in a Ca2+-dependent manner. IFT trains transiently pause through surface adhesion of their FMG cargos, and dynein-1b motors pull the cell towards the distal tip of the axoneme. Each train is transported by at least four motors, with only one type of motor active at a time. Our results demonstrate the mechanism of Chlamydomonas gliding motility and suggest that IFT plays a major role in adhesioninduced ciliary signaling pathways. Copyright Shih et al.

Original languageEnglish (US)
Article numbere00744
JournaleLife
Volume2013
Issue number2
DOIs
StatePublished - Jun 11 2013

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Shih, S. M., Engel, B. D., Kocabas, F., Bilyard, T., Gennerich, A., Marshall, W. F., & Yildiz, A. (2013). Intraflagellar transport drives flagellar surface motility. eLife, 2013(2), [e00744]. https://doi.org/10.7554/eLife.00744