Intracellular spermine prevents acid-induced uncoupling of Cx43 gap junction channels

Serguei N. Skatchkov, Feliksas F. Bukauskas, Jan Benedikt, Mikhail Inyushin, Yuriy V. Kucheryavykh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Polyamines (PAs), such as spermine and spermidine, modulate the activity of numerous receptors and channels in the central nervous system (CNS) and are stored in glial cells; however, little attention has been paid to their role in the regulation of connexin (Cx)-based gap junction channels. We have previously shown that PAs facilitate diffusion of Lucifer Yellow through astrocytic gap junctions in acute brain slices; therefore, we hypothesized that spermine can regulate Cx43-mediated (as the most abundant Cx in astrocytes) gap junctional communication. We used electrophysiological patch-clamp recording from paired Novikoff cells endogenously expressing Cx43 and HeLaCx43-EGFP transfectants to study pH-dependent modulation of cell-cell coupling in the presence or absence of PAs. Our results showed (i) a higher increase in gap junctional communication at higher concentrations of cytoplasmic spermine, and (ii) that spermine prevented uncoupling of gap junctions at low intracellular pH. Taken together, we conclude that spermine enhances Cx43-mediated gap junctional communication and may preserve neuronal excitability during ischemia and trauma when pH in the brain acidifies. We, therefore, suggest a new role of spermine in the regulation of a Cx43-based network under (patho)physiological conditions.

Original languageEnglish (US)
Pages (from-to)528-532
Number of pages5
JournalNeuroReport
Volume26
Issue number9
DOIs
StatePublished - Jun 9 2015

Fingerprint

Connexin 43
Spermine
Gap Junctions
Acids
Polyamines
Connexins
Spermidine
Brain
Neuroglia
Astrocytes
Ischemia
Central Nervous System
Wounds and Injuries

Keywords

  • Cx43 gap junction channels
  • polyamines
  • spermine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Skatchkov, S. N., Bukauskas, F. F., Benedikt, J., Inyushin, M., & Kucheryavykh, Y. V. (2015). Intracellular spermine prevents acid-induced uncoupling of Cx43 gap junction channels. NeuroReport, 26(9), 528-532. https://doi.org/10.1097/WNR.0000000000000385

Intracellular spermine prevents acid-induced uncoupling of Cx43 gap junction channels. / Skatchkov, Serguei N.; Bukauskas, Feliksas F.; Benedikt, Jan; Inyushin, Mikhail; Kucheryavykh, Yuriy V.

In: NeuroReport, Vol. 26, No. 9, 09.06.2015, p. 528-532.

Research output: Contribution to journalArticle

Skatchkov, SN, Bukauskas, FF, Benedikt, J, Inyushin, M & Kucheryavykh, YV 2015, 'Intracellular spermine prevents acid-induced uncoupling of Cx43 gap junction channels', NeuroReport, vol. 26, no. 9, pp. 528-532. https://doi.org/10.1097/WNR.0000000000000385
Skatchkov SN, Bukauskas FF, Benedikt J, Inyushin M, Kucheryavykh YV. Intracellular spermine prevents acid-induced uncoupling of Cx43 gap junction channels. NeuroReport. 2015 Jun 9;26(9):528-532. https://doi.org/10.1097/WNR.0000000000000385
Skatchkov, Serguei N. ; Bukauskas, Feliksas F. ; Benedikt, Jan ; Inyushin, Mikhail ; Kucheryavykh, Yuriy V. / Intracellular spermine prevents acid-induced uncoupling of Cx43 gap junction channels. In: NeuroReport. 2015 ; Vol. 26, No. 9. pp. 528-532.
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