TY - JOUR
T1 - Intracellular mRNA transport and localized translation
AU - Das, Sulagna
AU - Vera Ugalde, Maria
AU - Gandin, Valentina
AU - Singer, Robert H.
AU - Tutucci, Evelina
N1 - Funding Information:
The authors would like to thank the anonymous reviewers for the constructive comments and apologize to collaborators and colleagues whose work could not be cited due to space limitations. This work was supported by NIH grant AG05583 and NSERC grant RGPIN-2019-04767 to M.V., NIH grant NS083085 and NIH Grant GM57071 to R.H.S., and a Rose F. Kennedy IDDRC Pilot Grant to S.D.
Publisher Copyright:
© 2021, Springer Nature Limited.
PY - 2021/7
Y1 - 2021/7
N2 - Fine-tuning cellular physiology in response to intracellular and environmental cues requires precise temporal and spatial control of gene expression. High-resolution imaging technologies to detect mRNAs and their translation state have revealed that all living organisms localize mRNAs in subcellular compartments and create translation hotspots, enabling cells to tune gene expression locally. Therefore, mRNA localization is a conserved and integral part of gene expression regulation from prokaryotic to eukaryotic cells. In this Review, we discuss the mechanisms of mRNA transport and local mRNA translation across the kingdoms of life and at organellar, subcellular and multicellular resolution. We also discuss the properties of messenger ribonucleoprotein and higher order RNA granules and how they may influence mRNA transport and local protein synthesis. Finally, we summarize the technological developments that allow us to study mRNA localization and local translation through the simultaneous detection of mRNAs and proteins in single cells, mRNA and nascent protein single-molecule imaging, and bulk RNA and protein detection methods.
AB - Fine-tuning cellular physiology in response to intracellular and environmental cues requires precise temporal and spatial control of gene expression. High-resolution imaging technologies to detect mRNAs and their translation state have revealed that all living organisms localize mRNAs in subcellular compartments and create translation hotspots, enabling cells to tune gene expression locally. Therefore, mRNA localization is a conserved and integral part of gene expression regulation from prokaryotic to eukaryotic cells. In this Review, we discuss the mechanisms of mRNA transport and local mRNA translation across the kingdoms of life and at organellar, subcellular and multicellular resolution. We also discuss the properties of messenger ribonucleoprotein and higher order RNA granules and how they may influence mRNA transport and local protein synthesis. Finally, we summarize the technological developments that allow us to study mRNA localization and local translation through the simultaneous detection of mRNAs and proteins in single cells, mRNA and nascent protein single-molecule imaging, and bulk RNA and protein detection methods.
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U2 - 10.1038/s41580-021-00356-8
DO - 10.1038/s41580-021-00356-8
M3 - Review article
C2 - 33837370
AN - SCOPUS:85104134452
VL - 22
SP - 483
EP - 504
JO - Nature Reviews Molecular Cell Biology
JF - Nature Reviews Molecular Cell Biology
SN - 1471-0072
IS - 7
ER -