Intracellular localization of the migration inhibitory factor (Mif) in a long-term human lymphoid cell line1

Michael B. Prystowsky, Carol F. Sorokin, Walter S. Ceglowski, Kurt Hirschhorn, Philip R. Glade

Research output: Contribution to journalArticle

Abstract

Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: ß-glucuroni-dase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activtiy was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of esterase. No such correlation with MIF activity could be demonstrated for ß-glucuronidase or LDH.

Original languageEnglish (US)
Pages (from-to)1-2
Number of pages2
JournalInternational Archives of Allergy and Immunology
Volume48
Issue number2
DOIs
Publication statusPublished - Jan 1 1975
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this