Intracellular localization of the migration inhibitory factor (Mif) in a long-term human lymphoid cell line1

Michael B. Prystowsky; Carol F. Sorokin; Walter S. Ceglowski; Kurt Hirschhorn; Philip R. Glade

doi:10.1159/000231309

Intracellular localization of the migration inhibitory factor (Mif) in a long-term human lymphoid cell line1

Michael B. Prystowsky, Carol F. Sorokin, Walter S. Ceglowski, Kurt Hirschhorn, Philip R. Glade

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: ß-glucuroni-dase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activtiy was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of esterase. No such correlation with MIF activity could be demonstrated for ß-glucuronidase or LDH.

Original language	English (US)
Pages (from-to)	225-235
Number of pages	11
Journal	International Archives of Allergy and Immunology
Volume	48
Issue number	2
DOIs	https://doi.org/10.1159/000231309
State	Published - 1975
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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@article{ed9ad487543242fe9c58c852dfcb81fb,

title = "Intracellular localization of the migration inhibitory factor (Mif) in a long-term human lymphoid cell line1",

abstract = "Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: {\ss}-glucuroni-dase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activtiy was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of esterase. No such correlation with MIF activity could be demonstrated for {\ss}-glucuronidase or LDH.",

author = "Prystowsky, {Michael B.} and Sorokin, {Carol F.} and Ceglowski, {Walter S.} and Kurt Hirschhorn and Glade, {Philip R.}",

year = "1975",

doi = "10.1159/000231309",

language = "English (US)",

volume = "48",

pages = "225--235",

journal = "International Archives of Allergy and Immunology",

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T1 - Intracellular localization of the migration inhibitory factor (Mif) in a long-term human lymphoid cell line1

AU - Prystowsky, Michael B.

AU - Sorokin, Carol F.

AU - Ceglowski, Walter S.

AU - Hirschhorn, Kurt

AU - Glade, Philip R.

PY - 1975

Y1 - 1975

N2 - Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: ß-glucuroni-dase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activtiy was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of esterase. No such correlation with MIF activity could be demonstrated for ß-glucuronidase or LDH.

AB - Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: ß-glucuroni-dase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activtiy was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of esterase. No such correlation with MIF activity could be demonstrated for ß-glucuronidase or LDH.

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Intracellular localization of the migration inhibitory factor (Mif) in a long-term human lymphoid cell line1

Abstract

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