Subcellular fractions of the human lymphoid cell line PGLC 33H were obtained by N 2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: β glucuronidase for the lysosomal intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH (lactic dehydrogenase) for the supernatant fraction. These subcellular fractions were studied for MIF (migratory inhibition factor) activity, utilizing human lymphoid cells from established lines as target cells. MIF activity was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of esterase. No such correlation with MIF activity could be demonstrated for β glucuronidase or LDH.
|Original language||English (US)|
|Number of pages||11|
|Journal||International Archives of Allergy and Applied Immunology|
|State||Published - Jan 1 1975|
ASJC Scopus subject areas
- Immunology and Allergy