TY - JOUR
T1 - Intracellular localization of messenger RNAs for cytoskeletal proteins
AU - Lawrence, Jeanne Bentley
AU - Singer, Robert H.
N1 - Funding Information:
We wish to acknowledge Carol Villnave for her excellent technical contribution. We would also like to thank Marie Giorgio and Susan Billings-Gagliardi for patient help with photography, Roya Rashtchian for technical support, Elayn Byron for secretarial assistance, and Kevin Byron for sacrificing Saturday mornings to tissue culture. The actin and tubulin probes were from original clones by Don Cleveland and the vimen-tin probe was obtained through the generosity of Bruce Paterson. This work has been supported by grant HD18066 from the National Institutes of Health and an MDA fellowship to J. 8. L.
PY - 1986/5/9
Y1 - 1986/5/9
N2 - We have analyzed intracellular distributions of mRNAs for the cytoskeletal proteins actin, vimentin, and tubulin by in situ hybridization. Although polyadenylated RNA was homogeneously distributed throughout the cell, actin mRNA demonstrated a nonhomogeneous distribution in 95% of randomly selected chicken embryonic myoblasts and fibroblasts, as detected by isotopic and nonisotopic techniques. Actin mRNA concentrations were highest at cell extremities, generally in lamellipodia, where grain densities were up to 16-fold higher than in areas near the nucleus. Vimentin mRNA, unlike actin mRNA, was distributed near the nucleus. Tubulin mRNA appeared most concentrated in the peripheral cytoplasm. These results demonstrate that cytoplasmic mRNAs are localized in specific, nonrandom cellular patterns and that localized concentrations of specific proteins may result from corresponding localization of their respective mRNAs. Hence, actin mRNA distribution may result in increased concentration of actin filaments in lamellipodia of motile cells.
AB - We have analyzed intracellular distributions of mRNAs for the cytoskeletal proteins actin, vimentin, and tubulin by in situ hybridization. Although polyadenylated RNA was homogeneously distributed throughout the cell, actin mRNA demonstrated a nonhomogeneous distribution in 95% of randomly selected chicken embryonic myoblasts and fibroblasts, as detected by isotopic and nonisotopic techniques. Actin mRNA concentrations were highest at cell extremities, generally in lamellipodia, where grain densities were up to 16-fold higher than in areas near the nucleus. Vimentin mRNA, unlike actin mRNA, was distributed near the nucleus. Tubulin mRNA appeared most concentrated in the peripheral cytoplasm. These results demonstrate that cytoplasmic mRNAs are localized in specific, nonrandom cellular patterns and that localized concentrations of specific proteins may result from corresponding localization of their respective mRNAs. Hence, actin mRNA distribution may result in increased concentration of actin filaments in lamellipodia of motile cells.
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U2 - 10.1016/0092-8674(86)90326-0
DO - 10.1016/0092-8674(86)90326-0
M3 - Article
C2 - 3698103
AN - SCOPUS:0022497718
SN - 0092-8674
VL - 45
SP - 407
EP - 415
JO - Cell
JF - Cell
IS - 3
ER -