Intracellular localization of messenger RNAs for cytoskeletal proteins

Jeanne Bentley Lawrence, Robert H. Singer

Research output: Contribution to journalArticle

409 Citations (Scopus)

Abstract

We have analyzed intracellular distributions of mRNAs for the cytoskeletal proteins actin, vimentin, and tubulin by in situ hybridization. Although polyadenylated RNA was homogeneously distributed throughout the cell, actin mRNA demonstrated a nonhomogeneous distribution in 95% of randomly selected chicken embryonic myoblasts and fibroblasts, as detected by isotopic and nonisotopic techniques. Actin mRNA concentrations were highest at cell extremities, generally in lamellipodia, where grain densities were up to 16-fold higher than in areas near the nucleus. Vimentin mRNA, unlike actin mRNA, was distributed near the nucleus. Tubulin mRNA appeared most concentrated in the peripheral cytoplasm. These results demonstrate that cytoplasmic mRNAs are localized in specific, nonrandom cellular patterns and that localized concentrations of specific proteins may result from corresponding localization of their respective mRNAs. Hence, actin mRNA distribution may result in increased concentration of actin filaments in lamellipodia of motile cells.

Original languageEnglish (US)
Pages (from-to)407-415
Number of pages9
JournalCell
Volume45
Issue number3
DOIs
StatePublished - May 9 1986
Externally publishedYes

Fingerprint

Cytoskeletal Proteins
Messenger RNA
Actins
Pseudopodia
Vimentin
Tubulin
Myoblasts
Fibroblasts
Actin Cytoskeleton
In Situ Hybridization
Chickens
Cytoplasm
Extremities

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Intracellular localization of messenger RNAs for cytoskeletal proteins. / Lawrence, Jeanne Bentley; Singer, Robert H.

In: Cell, Vol. 45, No. 3, 09.05.1986, p. 407-415.

Research output: Contribution to journalArticle

Lawrence, Jeanne Bentley ; Singer, Robert H. / Intracellular localization of messenger RNAs for cytoskeletal proteins. In: Cell. 1986 ; Vol. 45, No. 3. pp. 407-415.
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