Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with Triton X-100

Jeffrey Pudney, Robert H. Singer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The method of Triton X-100 extraction and critical point drying of whole mounts of cultured chick and human myoblasts was used to study the presence of intracellular bundles of filaments within these cells. Observation by means of transmission and scanning electron microscopy demonstrated a complex system of filament bundles which appeared morphologically and spatially heterogeneous. Most obvious were long dense bundles or cables traversing along the ventral surface of developing myoblasts, presumably the 'stress fibers' seen in light microscopy. Other bundle types occurred which were composed of loose aggregates of filaments coursing through the remnant cell body. A prominent accumulation of filaments was also seen at the lateral edges of these myoblasts. These lateral edge cables were thicker and denser than any other type of filament bundle observed in the myoblasts. Reaction of unextracted myoblasts directly to human antiplatelet myosin conjugated to rhodamine demonstrated that the most intense reaction also occurred along the lateral edges of both human and chick myoblasts. During development of chick myoblasts the filament bundles became oriented parallel to the cell axis giving the cell a fusiform morphology. It is possible that the various filament bundle structures and their differing structural and spatial dispositions could be related to functional differences among the diverse population of intracellular bundles of filaments.

Original languageEnglish (US)
Pages (from-to)595-612
Number of pages18
JournalTissue and Cell
Volume12
Issue number4
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

myoblasts
Myoblasts
Octoxynol
chicks
cables (equipment)
cells
Stress Fibers
Scanning Transmission Electron Microscopy
Rhodamines
Myosins
cell aggregates
myosin
octoxynol
transmission electron microscopy
light microscopy
Microscopy
scanning electron microscopy
drying
Observation
Light

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

Cite this

Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with Triton X-100. / Pudney, Jeffrey; Singer, Robert H.

In: Tissue and Cell, Vol. 12, No. 4, 1980, p. 595-612.

Research output: Contribution to journalArticle

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