Intestinal crypts recover rapidly from focal damage with coordinated motion of stem cells that is impaired by aging

Jiahn Choi, Nikolai Rakhilin, Poornima Gadamsetty, Daniel J. Joe, Tahmineh Tabrizian, Steven M. Lipkin, Derek M. Huffman, Xiling Shen, Nozomi Nishimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Despite the continuous renewal and turnover of the small intestinal epithelium, the intestinal crypt maintains a ‘soccer ball-like’, alternating pattern of stem and Paneth cells at the base of the crypt. To study the robustness of the alternating pattern, we used intravital two-photon microscopy in mice with fluorescently-labeled Lgr5+ intestinal stem cells and precisely perturbed the mosaic pattern with femtosecond laser ablation. Ablation of one to three cells initiated rapid motion of crypt cells that restored the alternation in the pattern within about two hours with only the rearrangement of pre-existing cells, without any cell division. Crypt cells then performed a coordinated dilation of the crypt lumen, which resulted in peristalsis-like motion that forced damaged cells out of the crypt. Crypt cell motion was reduced with inhibition of the ROCK pathway and attenuated with old age, and both resulted in incomplete pattern recovery. This suggests that in addition to proliferation and self-renewal, motility of stem cells is critical for maintaining homeostasis. Reduction of this newly-identified behavior of stem cells could contribute to disease and age-related changes.

Original languageEnglish (US)
Article number10989
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Stem Cells
Paneth Cells
Peristalsis
Soccer
Laser Therapy
Intestinal Mucosa
Photons
Cell Division
Dilatation
Microscopy
Homeostasis

ASJC Scopus subject areas

  • General

Cite this

Choi, J., Rakhilin, N., Gadamsetty, P., Joe, D. J., Tabrizian, T., Lipkin, S. M., ... Nishimura, N. (2018). Intestinal crypts recover rapidly from focal damage with coordinated motion of stem cells that is impaired by aging. Scientific Reports, 8(1), [10989]. https://doi.org/10.1038/s41598-018-29230-y

Intestinal crypts recover rapidly from focal damage with coordinated motion of stem cells that is impaired by aging. / Choi, Jiahn; Rakhilin, Nikolai; Gadamsetty, Poornima; Joe, Daniel J.; Tabrizian, Tahmineh; Lipkin, Steven M.; Huffman, Derek M.; Shen, Xiling; Nishimura, Nozomi.

In: Scientific Reports, Vol. 8, No. 1, 10989, 01.12.2018.

Research output: Contribution to journalArticle

Choi, J, Rakhilin, N, Gadamsetty, P, Joe, DJ, Tabrizian, T, Lipkin, SM, Huffman, DM, Shen, X & Nishimura, N 2018, 'Intestinal crypts recover rapidly from focal damage with coordinated motion of stem cells that is impaired by aging', Scientific Reports, vol. 8, no. 1, 10989. https://doi.org/10.1038/s41598-018-29230-y
Choi, Jiahn ; Rakhilin, Nikolai ; Gadamsetty, Poornima ; Joe, Daniel J. ; Tabrizian, Tahmineh ; Lipkin, Steven M. ; Huffman, Derek M. ; Shen, Xiling ; Nishimura, Nozomi. / Intestinal crypts recover rapidly from focal damage with coordinated motion of stem cells that is impaired by aging. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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