Intestinal alkaline phosphatase administration in newborns decreases systemic inflammatory cytokine expression in a neonatal necrotizing enterocolitis rat model

Rebecca M. Rentea, Jennifer L. Liedel, Katherine Fredrich, Scott R. Welak, Kirkwood A. Pritchard, Keith T. Oldham, Pippa M. Simpson, David M. Gourlay

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Supplementation of intestinal alkaline phosphatase (IAP), an endogenous protein expressed in the intestines, decreases the severity of necrotizing enterocolitis (NEC)-associated intestinal injury and permeability. We hypothesized that IAP administration is protective in a dose-dependent manner of the inflammatory response in a neonatal rat model. Materials and methods: Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on day of life 3. Control pups were vaginally delivered and dam fed. Preterm pups were delivered via cesarean section and exposed to intermittent hypoxia and formula feeds containing lipopolysaccharide (NEC) with and without IAP. Three different standardized doses were administered to a group of pups treated with 40, 4, and 0.4 U/kg of bovine IAP (NEC + IAP40, IAP4, or IAP0.4 U). Reverse transcription-real-time polymerase chain reaction (RT-PCR) for inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α on liver and lung tissues and serum cytokine analysis for interleukin (IL)-1β, IL-6, IL-10, and TNF-α were performed. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests, expressed as mean ± standard error of the mean and P ≤ 0.05 considered significant. Results: Levels of cytokines IL-1β, IL-6, and TNF-α increased significantly in NEC versus control, returning to control levels with increasing doses of supplemental enteral IAP. Hepatic and pulmonary TNF-α and iNOS messenger ribonucleic acid expressions increased in NEC, and the remaining elevated despite IAP supplementation. Conclusions: Proinflammatory cytokine expression is increased systemically with intestinal NEC injury. Administration of IAP significantly reduces systemic proinflammatory cytokine expression in a dose-dependent manner. Early supplemental enteral IAP may reduce NEC-related injury and be useful for reducing effects caused by a proinflammatory cascade.

Original languageEnglish (US)
Pages (from-to)228-234
Number of pages7
JournalJournal of Surgical Research
Volume177
Issue number2
DOIs
StatePublished - Oct 2012
Externally publishedYes

Fingerprint

Necrotizing Enterocolitis
Alkaline Phosphatase
Cytokines
Tumor Necrosis Factor-alpha
Nitric Oxide Synthase Type II
Interleukin-1
Small Intestine
Interleukin-6
Wounds and Injuries
Lung
Liver
Cesarean Section
Interleukin-10
Reverse Transcription
Intestines
Sprague Dawley Rats
Lipopolysaccharides
Real-Time Polymerase Chain Reaction
Permeability
RNA

Keywords

  • Cytokine
  • Intestinal alkaline phosphatase
  • Necrotizing enterocolitis
  • Systemic inflammation

ASJC Scopus subject areas

  • Surgery

Cite this

Intestinal alkaline phosphatase administration in newborns decreases systemic inflammatory cytokine expression in a neonatal necrotizing enterocolitis rat model. / Rentea, Rebecca M.; Liedel, Jennifer L.; Fredrich, Katherine; Welak, Scott R.; Pritchard, Kirkwood A.; Oldham, Keith T.; Simpson, Pippa M.; Gourlay, David M.

In: Journal of Surgical Research, Vol. 177, No. 2, 10.2012, p. 228-234.

Research output: Contribution to journalArticle

Rentea, Rebecca M. ; Liedel, Jennifer L. ; Fredrich, Katherine ; Welak, Scott R. ; Pritchard, Kirkwood A. ; Oldham, Keith T. ; Simpson, Pippa M. ; Gourlay, David M. / Intestinal alkaline phosphatase administration in newborns decreases systemic inflammatory cytokine expression in a neonatal necrotizing enterocolitis rat model. In: Journal of Surgical Research. 2012 ; Vol. 177, No. 2. pp. 228-234.
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abstract = "Background: Supplementation of intestinal alkaline phosphatase (IAP), an endogenous protein expressed in the intestines, decreases the severity of necrotizing enterocolitis (NEC)-associated intestinal injury and permeability. We hypothesized that IAP administration is protective in a dose-dependent manner of the inflammatory response in a neonatal rat model. Materials and methods: Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on day of life 3. Control pups were vaginally delivered and dam fed. Preterm pups were delivered via cesarean section and exposed to intermittent hypoxia and formula feeds containing lipopolysaccharide (NEC) with and without IAP. Three different standardized doses were administered to a group of pups treated with 40, 4, and 0.4 U/kg of bovine IAP (NEC + IAP40, IAP4, or IAP0.4 U). Reverse transcription-real-time polymerase chain reaction (RT-PCR) for inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α on liver and lung tissues and serum cytokine analysis for interleukin (IL)-1β, IL-6, IL-10, and TNF-α were performed. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests, expressed as mean ± standard error of the mean and P ≤ 0.05 considered significant. Results: Levels of cytokines IL-1β, IL-6, and TNF-α increased significantly in NEC versus control, returning to control levels with increasing doses of supplemental enteral IAP. Hepatic and pulmonary TNF-α and iNOS messenger ribonucleic acid expressions increased in NEC, and the remaining elevated despite IAP supplementation. Conclusions: Proinflammatory cytokine expression is increased systemically with intestinal NEC injury. Administration of IAP significantly reduces systemic proinflammatory cytokine expression in a dose-dependent manner. Early supplemental enteral IAP may reduce NEC-related injury and be useful for reducing effects caused by a proinflammatory cascade.",
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AU - Rentea, Rebecca M.

AU - Liedel, Jennifer L.

AU - Fredrich, Katherine

AU - Welak, Scott R.

AU - Pritchard, Kirkwood A.

AU - Oldham, Keith T.

AU - Simpson, Pippa M.

AU - Gourlay, David M.

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N2 - Background: Supplementation of intestinal alkaline phosphatase (IAP), an endogenous protein expressed in the intestines, decreases the severity of necrotizing enterocolitis (NEC)-associated intestinal injury and permeability. We hypothesized that IAP administration is protective in a dose-dependent manner of the inflammatory response in a neonatal rat model. Materials and methods: Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on day of life 3. Control pups were vaginally delivered and dam fed. Preterm pups were delivered via cesarean section and exposed to intermittent hypoxia and formula feeds containing lipopolysaccharide (NEC) with and without IAP. Three different standardized doses were administered to a group of pups treated with 40, 4, and 0.4 U/kg of bovine IAP (NEC + IAP40, IAP4, or IAP0.4 U). Reverse transcription-real-time polymerase chain reaction (RT-PCR) for inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α on liver and lung tissues and serum cytokine analysis for interleukin (IL)-1β, IL-6, IL-10, and TNF-α were performed. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests, expressed as mean ± standard error of the mean and P ≤ 0.05 considered significant. Results: Levels of cytokines IL-1β, IL-6, and TNF-α increased significantly in NEC versus control, returning to control levels with increasing doses of supplemental enteral IAP. Hepatic and pulmonary TNF-α and iNOS messenger ribonucleic acid expressions increased in NEC, and the remaining elevated despite IAP supplementation. Conclusions: Proinflammatory cytokine expression is increased systemically with intestinal NEC injury. Administration of IAP significantly reduces systemic proinflammatory cytokine expression in a dose-dependent manner. Early supplemental enteral IAP may reduce NEC-related injury and be useful for reducing effects caused by a proinflammatory cascade.

AB - Background: Supplementation of intestinal alkaline phosphatase (IAP), an endogenous protein expressed in the intestines, decreases the severity of necrotizing enterocolitis (NEC)-associated intestinal injury and permeability. We hypothesized that IAP administration is protective in a dose-dependent manner of the inflammatory response in a neonatal rat model. Materials and methods: Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on day of life 3. Control pups were vaginally delivered and dam fed. Preterm pups were delivered via cesarean section and exposed to intermittent hypoxia and formula feeds containing lipopolysaccharide (NEC) with and without IAP. Three different standardized doses were administered to a group of pups treated with 40, 4, and 0.4 U/kg of bovine IAP (NEC + IAP40, IAP4, or IAP0.4 U). Reverse transcription-real-time polymerase chain reaction (RT-PCR) for inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α on liver and lung tissues and serum cytokine analysis for interleukin (IL)-1β, IL-6, IL-10, and TNF-α were performed. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests, expressed as mean ± standard error of the mean and P ≤ 0.05 considered significant. Results: Levels of cytokines IL-1β, IL-6, and TNF-α increased significantly in NEC versus control, returning to control levels with increasing doses of supplemental enteral IAP. Hepatic and pulmonary TNF-α and iNOS messenger ribonucleic acid expressions increased in NEC, and the remaining elevated despite IAP supplementation. Conclusions: Proinflammatory cytokine expression is increased systemically with intestinal NEC injury. Administration of IAP significantly reduces systemic proinflammatory cytokine expression in a dose-dependent manner. Early supplemental enteral IAP may reduce NEC-related injury and be useful for reducing effects caused by a proinflammatory cascade.

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KW - Necrotizing enterocolitis

KW - Systemic inflammation

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