Interrater agreement of anal cytology

Teresa M. Darragh, Diane Tokugawa, Philip E. Castle, Stephen Follansbee, Sylvia Borgonovo, Brandon J. LaMere, Lauren Schwartz, Julia C. Gage, Barbara Fetterman, Thomas Lorey, Nicolas Wentzensen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

BACKGROUND: The majority of anal cancers are caused by persistent infections with carcinogenic human papillomaviruses (HPV). Similar to cervical carcinogenesis, the progression from HPV infection to anal cancer occurs through precancerous lesions that can be treated to prevent invasion. In analogy to cervical cytology, anal cytology has been proposed as a screening tool for anal cancer precursors in high-risk populations. METHODS: The authors analyzed the interobserver reproducibility of anal cytology in a population of 363 human immunodeficiency virus (HIV)-infected men who have sex with men (MSM). Liquid-based cytology (LBC) specimens were collected in the anal dysplasia clinic before the performance of high-resolution anoscopy on all patients. Papanicolaou-stained LBC slides were evaluated by 2 cytopathologists, each of whom was blinded to the clinical outcome and the other pathologist's results, using the revised Bethesda terminology. RESULTS: Overall agreement between the 2 observers was 66% (kappa, 0.54; linear-weighted kappa, 0.69). Using dichotomizing cytology results (atypical squamous cells of undetermined significance [ASC-US] or worse vs less than ASC-US), the agreement increased to 86% (kappa, 0.69). An increasing likelihood of testing positive for markers associated with HPV-related transformation, p16/Ki-67, and HPV oncogene messenger RNA was observed, with increasing severity of cytology results noted both for individual cytologists and for consensus cytology interpretation (P value for trend [ptrend] < .0001 for all). CONCLUSIONS: Moderate to good agreement was observed between 2 cytopathologists evaluating anal cytology samples collected from HIV-positive MSM. A higher severity of anal cytology was associated with biomarkers of anal precancerous lesions. Anal cytology may be used for anal cancer screening in high-risk populations, and biomarkers of HPV-related transformation can serve as quality control for anal cytology.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalCancer cytopathology
Volume121
Issue number2
DOIs
StatePublished - Feb 2013
Externally publishedYes

Fingerprint

Cell Biology
Anus Neoplasms
Biomarkers
HIV
Population
Papillomavirus Infections
Oncogenes
Early Detection of Cancer
Terminology
Quality Control
Carcinogenesis
Messenger RNA

Keywords

  • Anal cancer screening
  • Biomarkers
  • Cytology
  • Human immunodeficiency virus (HIV)
  • Human papillomavirus (HPV)
  • Men who have sex with men (MSM)

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Darragh, T. M., Tokugawa, D., Castle, P. E., Follansbee, S., Borgonovo, S., LaMere, B. J., ... Wentzensen, N. (2013). Interrater agreement of anal cytology. Cancer cytopathology, 121(2), 72-78. https://doi.org/10.1002/cncy.21218

Interrater agreement of anal cytology. / Darragh, Teresa M.; Tokugawa, Diane; Castle, Philip E.; Follansbee, Stephen; Borgonovo, Sylvia; LaMere, Brandon J.; Schwartz, Lauren; Gage, Julia C.; Fetterman, Barbara; Lorey, Thomas; Wentzensen, Nicolas.

In: Cancer cytopathology, Vol. 121, No. 2, 02.2013, p. 72-78.

Research output: Contribution to journalArticle

Darragh, TM, Tokugawa, D, Castle, PE, Follansbee, S, Borgonovo, S, LaMere, BJ, Schwartz, L, Gage, JC, Fetterman, B, Lorey, T & Wentzensen, N 2013, 'Interrater agreement of anal cytology', Cancer cytopathology, vol. 121, no. 2, pp. 72-78. https://doi.org/10.1002/cncy.21218
Darragh TM, Tokugawa D, Castle PE, Follansbee S, Borgonovo S, LaMere BJ et al. Interrater agreement of anal cytology. Cancer cytopathology. 2013 Feb;121(2):72-78. https://doi.org/10.1002/cncy.21218
Darragh, Teresa M. ; Tokugawa, Diane ; Castle, Philip E. ; Follansbee, Stephen ; Borgonovo, Sylvia ; LaMere, Brandon J. ; Schwartz, Lauren ; Gage, Julia C. ; Fetterman, Barbara ; Lorey, Thomas ; Wentzensen, Nicolas. / Interrater agreement of anal cytology. In: Cancer cytopathology. 2013 ; Vol. 121, No. 2. pp. 72-78.
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AU - LaMere, Brandon J.

AU - Schwartz, Lauren

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