Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis

Graziella Migliorati, Lldo Nicoletti, M. Cristina Pagliacci, Luciano D'Adamio, Carlo Riccardi

Research output: Contribution to journalArticle

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Abstract

Glucocorticoid hormones (GCH) and anti-CD3 monoclonal antibodies (MoAbs) induce in mouse thymocytes and T-cell tumor lines an active process of cell death called apoptosis. Interleukins (IL), including IL-1 and IL-2, have been reported to inhibit such apoptosis. In this study we show that IL-4 also reduced the DNA fragmentation characteristic of dexamethasone (DEX)-induced apoptosis in thymocytes. This effect, studied in both time-course and dose-response experiments, was also detected at low IL-4 concentrations (1 U/mL) and against high DEX levels (10-7 mol/L). The effect of IL-4 was blocked by an anti-IL-4 but not by an anti-IL-1α MoAb, and was thus both specific and direct. Phenotypic analysis showed that IL-4 protects predominantly CD4-CD8- and CD4+CD8- cells. Our findings suggest that intrathymic T-cell development may be influenced by IL-4.

Original languageEnglish (US)
Pages (from-to)1352-1358
Number of pages7
JournalBlood
Volume81
Issue number5
StatePublished - Mar 1 1993
Externally publishedYes

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Thymocytes
Interleukin-4
Dexamethasone
Apoptosis
T-cells
Interleukin-1
T-Lymphocytes
Interleukins
DNA Fragmentation
Cell death
Glucocorticoids
Interleukin-2
Tumors
Cell Death
Monoclonal Antibodies
Hormones
Cell Line
DNA
Neoplasms
Experiments

ASJC Scopus subject areas

  • Hematology

Cite this

Migliorati, G., Nicoletti, L., Pagliacci, M. C., D'Adamio, L., & Riccardi, C. (1993). Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis. Blood, 81(5), 1352-1358.

Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis. / Migliorati, Graziella; Nicoletti, Lldo; Pagliacci, M. Cristina; D'Adamio, Luciano; Riccardi, Carlo.

In: Blood, Vol. 81, No. 5, 01.03.1993, p. 1352-1358.

Research output: Contribution to journalArticle

Migliorati, G, Nicoletti, L, Pagliacci, MC, D'Adamio, L & Riccardi, C 1993, 'Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis', Blood, vol. 81, no. 5, pp. 1352-1358.
Migliorati G, Nicoletti L, Pagliacci MC, D'Adamio L, Riccardi C. Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis. Blood. 1993 Mar 1;81(5):1352-1358.
Migliorati, Graziella ; Nicoletti, Lldo ; Pagliacci, M. Cristina ; D'Adamio, Luciano ; Riccardi, Carlo. / Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis. In: Blood. 1993 ; Vol. 81, No. 5. pp. 1352-1358.
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