Interleukin-4 protects double-negative and CD4 single-positive thymocytes from dexamethasone-induced apoptosis

G. Migliorati, I. Nicoletti, M. C. Pagliacci, L. D'Adamio, C. Riccardi

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Glucocorticoid hormones (GCH) and anti-CD3 monoclonal antibodies (MoAbs) induce in mouse thymocytes and T-cell tumor lines an active process of cell death called apoptosis. Interleukins (IL), including IL-1 and IL-2, have been reported to inhibit such apoptosis. In this study we show that IL-4 also reduced the DNA fragmentation characteristic of dexamethasone (DEX)-induced apoptosis in thymocytes. This effect, studied in both time-course and dose- response experiments, was also detected at low IL-4 concentrations (1 U/mL) and against high DEX levels (10-7 mol/L). The effect of IL-4 was blocked by an anti-IL-4 but not by an anti-IL-1α MoAb, and was thus both specific and direct. Phenotypic analysis showed that IL-4 protects predominantly CD4- CD8- and CD4+CD8- cells. Our findings suggest that intrathymic T-cell development may be influenced by IL-4.

Original languageEnglish (US)
Pages (from-to)1352-1358
Number of pages7
JournalBlood
Volume81
Issue number5
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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