Interleukin 4 alters human bone marrow stroma and modulates its interaction with hematopoietic progenitors

A. Ferrajoli, M. Talpaz, N. G. Ordonez, E. R. Stanley, C. Hirsch-Ginsberg, T. F. Zipf, M. Wetzler, R. Kurzrock, Z. Estrov

Research output: Contribution to journalArticle

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Abstract

To investigate the functional activity of interleukin 4 (IL-4) on human marrow stroma formation, normal bone marrow (BM) samples were cultured in 'Dexter-type' long-term cultures in the presence and absence of IL-4. IL-4 (0.001 to 1.0 μg/ml) added at the initiation of culture and once weekly when the cultures were fed effaced the culture architecture. In four-week old confluent cultures smooth muscle-like and endothelial-like cells were rare, the fibronectin network and cobblestone areas were absent, and a preponderance of monocyte-macrophages characterized the adherent layer. Exposure to IL-4 reduced the numbers of CD34+ cells, colony-forming unit granulocyte-macrophage (GFU-GM) cells and burst-forming unit-erythroid (BFU- E) cells in the adherent layer, and increased their numbers in the nonadherent layer. In five of eight IL-4-containing cultures the concentrations of macrophage colony-stimulating factor (M-CSF) were increased and in two of eight IL-4-treated cultures the concentrations of tumor necrosis factor-α (TNF-α) were significantly elevated as compared to those in control cultures, whereas there were no consistent differences in the levels of either IL-6 or transforming growth factor-β (TGF-β). IL-1β and granulocyte-macrophage CSF (GM-CSF) were not detected in any culture. These data suggest that IL-4 suppresses stroma formation and alters its structure and cellular composition.

Original languageEnglish (US)
Pages (from-to)638-649
Number of pages12
JournalStem Cells
Volume12
Issue number6
StatePublished - 1994
Externally publishedYes

Fingerprint

Interleukin-4
Bone Marrow
Macrophages
Granulocyte-Macrophage Progenitor Cells
Erythroid Cells
Macrophage Colony-Stimulating Factor
Transforming Growth Factors
Cellular Structures
Interleukin-1
Fibronectins
Granulocytes
Smooth Muscle
Monocytes
Interleukin-6
Endothelial Cells
Tumor Necrosis Factor-alpha
Cell Count

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ferrajoli, A., Talpaz, M., Ordonez, N. G., Stanley, E. R., Hirsch-Ginsberg, C., Zipf, T. F., ... Estrov, Z. (1994). Interleukin 4 alters human bone marrow stroma and modulates its interaction with hematopoietic progenitors. Stem Cells, 12(6), 638-649.

Interleukin 4 alters human bone marrow stroma and modulates its interaction with hematopoietic progenitors. / Ferrajoli, A.; Talpaz, M.; Ordonez, N. G.; Stanley, E. R.; Hirsch-Ginsberg, C.; Zipf, T. F.; Wetzler, M.; Kurzrock, R.; Estrov, Z.

In: Stem Cells, Vol. 12, No. 6, 1994, p. 638-649.

Research output: Contribution to journalArticle

Ferrajoli, A, Talpaz, M, Ordonez, NG, Stanley, ER, Hirsch-Ginsberg, C, Zipf, TF, Wetzler, M, Kurzrock, R & Estrov, Z 1994, 'Interleukin 4 alters human bone marrow stroma and modulates its interaction with hematopoietic progenitors', Stem Cells, vol. 12, no. 6, pp. 638-649.
Ferrajoli A, Talpaz M, Ordonez NG, Stanley ER, Hirsch-Ginsberg C, Zipf TF et al. Interleukin 4 alters human bone marrow stroma and modulates its interaction with hematopoietic progenitors. Stem Cells. 1994;12(6):638-649.
Ferrajoli, A. ; Talpaz, M. ; Ordonez, N. G. ; Stanley, E. R. ; Hirsch-Ginsberg, C. ; Zipf, T. F. ; Wetzler, M. ; Kurzrock, R. ; Estrov, Z. / Interleukin 4 alters human bone marrow stroma and modulates its interaction with hematopoietic progenitors. In: Stem Cells. 1994 ; Vol. 12, No. 6. pp. 638-649.
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