TY - JOUR
T1 - Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1
AU - Zhou, Y. Q.
AU - Stanley, E. R.
AU - Clark, S. C.
AU - Hatzfeld, J. A.
AU - Levesque, J. P.
AU - Federici, C.
AU - Watt, S. M.
AU - Hatzfeld, A.
PY - 1988
Y1 - 1988
N2 - By using human bone marrow cells enriched for early progenitors by selective immunoadsorption and plated at low cell density (103 to 104 cells/mL/9.6 cm2) in semisolid methylcellulose culture, we have analyzed the cooperative effects of human colony-stimulating factor 1 (CSF-1), granulocyte-macrophage-CSF (GM-CSF), interleukin-1α (IL-1α), and gibbon as well as human recombinant IL-3 on the formation of monocytic colonies. CSF-1 alone stimulated mature monocytic colony formation by human CFU-M. However, in the presence of IL-3 and erythropoietin, CSF-1 stimulated maximal immature monocytic colony formation at low concentrations and inhibited the formation of granulomonocytic, erythrocytic, and mixed colonies. Cultures with CSF-1 and IL-3 contained more immature monocytic colonies than did cultures with CSF-1 alone. IL-1α alone had little effect. However, IL-1α in combination with optimal concentrations of either CSF-1, GM-CSF, or IL-3 increased the number of colonies containing immature or mature monocytic colonies.
AB - By using human bone marrow cells enriched for early progenitors by selective immunoadsorption and plated at low cell density (103 to 104 cells/mL/9.6 cm2) in semisolid methylcellulose culture, we have analyzed the cooperative effects of human colony-stimulating factor 1 (CSF-1), granulocyte-macrophage-CSF (GM-CSF), interleukin-1α (IL-1α), and gibbon as well as human recombinant IL-3 on the formation of monocytic colonies. CSF-1 alone stimulated mature monocytic colony formation by human CFU-M. However, in the presence of IL-3 and erythropoietin, CSF-1 stimulated maximal immature monocytic colony formation at low concentrations and inhibited the formation of granulomonocytic, erythrocytic, and mixed colonies. Cultures with CSF-1 and IL-3 contained more immature monocytic colonies than did cultures with CSF-1 alone. IL-1α alone had little effect. However, IL-1α in combination with optimal concentrations of either CSF-1, GM-CSF, or IL-3 increased the number of colonies containing immature or mature monocytic colonies.
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U2 - 10.1182/blood.v72.6.1870.bloodjournal7261870
DO - 10.1182/blood.v72.6.1870.bloodjournal7261870
M3 - Article
C2 - 3264191
AN - SCOPUS:0024244052
SN - 0006-4971
VL - 72
SP - 1870
EP - 1874
JO - Blood
JF - Blood
IS - 6
ER -