Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1

Y. Q. Zhou, E. R. Stanley, S. C. Clark, J. A. Hatzfeld, J. P. Levesque, C. Federici, S. M. Watt, A. Hatzfeld

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Abstract

By using human bone marrow cells enriched for early progenitors by selective immunoadsorption and plated at low cell density (103 to 104 cells/mL/9.6 cm2) in semisolid methylcellulose culture, we have analyzed the cooperative effects of human colony-stimulating factor 1 (CSF-1), granulocyte-macrophage-CSF (GM-CSF), interleukin-1α (IL-1α), and gibbon as well as human recombinant IL-3 on the formation of monocytic colonies. CSF-1 alone stimulated mature monocytic colony formation by human CFU-M. However, in the presence of IL-3 and erythropoietin, CSF-1 stimulated maximal immature monocytic colony formation at low concentrations and inhibited the formation of granulomonocytic, erythrocytic, and mixed colonies. Cultures with CSF-1 and IL-3 contained more immature monocytic colonies than did cultures with CSF-1 alone. IL-1α alone had little effect. However, IL-1α in combination with optimal concentrations of either CSF-1, GM-CSF, or IL-3 increased the number of colonies containing immature or mature monocytic colonies.

Original languageEnglish (US)
Pages (from-to)1870-1874
Number of pages5
JournalBlood
Volume72
Issue number6
StatePublished - 1988
Externally publishedYes

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Macrophage Colony-Stimulating Factor
Interleukin-3
Interleukin-1
Bone
Bone Marrow
Macrophages
Granulocytes
Hylobates
Methylcellulose
Erythropoietin
Bone Marrow Cells
Cells
Cell Count

ASJC Scopus subject areas

  • Hematology

Cite this

Zhou, Y. Q., Stanley, E. R., Clark, S. C., Hatzfeld, J. A., Levesque, J. P., Federici, C., ... Hatzfeld, A. (1988). Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1. Blood, 72(6), 1870-1874.

Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1. / Zhou, Y. Q.; Stanley, E. R.; Clark, S. C.; Hatzfeld, J. A.; Levesque, J. P.; Federici, C.; Watt, S. M.; Hatzfeld, A.

In: Blood, Vol. 72, No. 6, 1988, p. 1870-1874.

Research output: Contribution to journalArticle

Zhou, YQ, Stanley, ER, Clark, SC, Hatzfeld, JA, Levesque, JP, Federici, C, Watt, SM & Hatzfeld, A 1988, 'Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1', Blood, vol. 72, no. 6, pp. 1870-1874.
Zhou YQ, Stanley ER, Clark SC, Hatzfeld JA, Levesque JP, Federici C et al. Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1. Blood. 1988;72(6):1870-1874.
Zhou, Y. Q. ; Stanley, E. R. ; Clark, S. C. ; Hatzfeld, J. A. ; Levesque, J. P. ; Federici, C. ; Watt, S. M. ; Hatzfeld, A. / Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1. In: Blood. 1988 ; Vol. 72, No. 6. pp. 1870-1874.
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