Interleukin-2 induces apoptosis in mouse thymocytes

Graziella Migliorati, Ildo Nicoletti, Maria Cristina Pagliacci, Luciano D’Adamio, Carlo Riccardi

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Abstract

Interleukins play a role in the process of T-cell development and, like other cytokines, seem able to modulate apoptosis. Interleukin-2 has been reported to inhibit apoptotic cell death of thymocytes induced in vitro by either activation of CD3/TCR complex or treatmet with glu-cocorticoid hormone. We demonstrate here that IL-2 can provoke DNA fragmentation and cell death of CD4+CD8+ mouse thymocytes by activating an endogenous apoptotic pathway. Thymocytes, incubated with high IL-2 concentrations in vitro, showed the morphological characteristics of apoptotic cells, including, reduction in nuclear size, derangement in chromatin structure, and DNA fragmentation in oligonucleosomal subunits. Inhibition of mRNA and protein synthesis and addition of the PKC-inhibitor H-7, Zn2+ ions, and IL-4 counteracted the IL-2 effect. These data suggest that high IL-2 concentrations may induce an active process of cell death on mouse thymocytes in vitro.

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalCellular Immunology
Volume146
Issue number1
DOIs
Publication statusPublished - Jan 1993

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ASJC Scopus subject areas

  • Immunology

Cite this

Migliorati, G., Nicoletti, I., Pagliacci, M. C., D’Adamio, L., & Riccardi, C. (1993). Interleukin-2 induces apoptosis in mouse thymocytes. Cellular Immunology, 146(1), 52-61. https://doi.org/10.1006/cimm.1993.1005